Biopolym. Cell. 2011; 27(5):381-382.
http://dx.doi.org/10.7124/bc.000128
Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol
1Kobbi L., 1Dias J., 1Octobre G., 1Comisso M., 1Kaminska M., 1, 2Shalak V. F., 1Mirande M.
  1. CNRS
    1, Ave. de la Terrasse, Gif-sur-Yvette, France, 91190
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA3Lys:GagPol packaging complex is proposed.
Keywords: tRNA3Lys, lysyl-tRNA synthetase, HIV-1, packaging
Full text: (PDF, in English)

References

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