Biopolym. Cell. 2011; 27(4):279-284.
http://dx.doi.org/10.7124/bc.00010A
Structure and Function of Biopolymers
Expression of the subunits of multiprotein translation elongation complex eEF1H in human renal carcinomas
1Veremieva M. V., 2Zgonnik Yu. M., 1Negrutskii B. S., 1El'skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. State Institution «Institute of Urology of NAMS of Ukraine»
    9-a, Yu. Kotsubyns'koho Str., Kyiv, Ukraine, 04053

Abstract

Aim. To investigate the expression of all eEF1 complex subunits at the mRNA and protein levels in human renal cancer. Methods. The protein content and mRNA level of the eEF1 complex components were analyzed in 18 clinical human renal carcinoma specimens and correspondingly paired normal tissues by Western blot and Northern blot techniques. Results. The changes in the level of protein components of the eEF1 complex or mRNAs coding for these proteins in human renal carcinoma specimens were found to be not essential. Conclusions. This study suggests that eEF1 subunits have no additional cancer-related functions in renal tissue. The data indicate that the occurrence of cancer-induced changes in the eEF1 subunits expression may depend on the tumor localization.
Keywords: protein synthesis, translation elongation factor eEF1H, human renal cell carcimoma
Full text: (PDF, in Ukrainian)

References

[1] Stumpf C. R., Ruggero D. The cancerous translation apparatus Curr. Opin. Genet. Dev 2011 21(4):474-83.
[2] Negrutskii B. S., El'skaya A. V. Eukaryotic translation elongation factor 1alpha: structure, expression, functions, and possible role in aminoacyl-tRNA channeling Prog. Nucl. Acid Res. Mol. Biol 1998 60 P. 47–78.
[3] Prokaryotic and eukaryotic translation factors. Ad Hoc Nomenclature Subcommittee Report Biochimie 1996 78, N 11– 12 P. 1119–1122.
[4] Liu G., Grant W. M., Persky D., Latham V. M. Jr., Singer R. H., Condeelis J. Interactions of elongation factor 1a with F-actin and b-actin mRNA: implications for anchoring mRNA in cell protrusions Mol. Biol. Cell 2002 13, N 2 P. 579–592.
[5] Chuang S. M., Chen L., Lambertson D., Anand M., Kinzy T. G., Madura K. Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A Mol. Cell. Biol 2005 25, N 1 P. 403–413.
[6] Ogawa K., Utsunomiya T., Mimori K., Tanaka Y., Tanaka F., Inoue H., Murayama S., Mori M. Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma Br. J. Cancer 2004 91, N 2 P. 282–286.
[7] Al-Maghrebi M., Anim J. T., Olalu A. A. Up-regulation of eukaryotic elongation factor-1 subunits in breast carcinoma Anticancer Res 2005 25, N 3c P. 2573–2577.
[8] Veremieva M. V., Shostak K. O., Malysheva T. A., Zozulya Y. P., Rozumenko V. D., Kavsan V. M., Negrutskii B. S. Investigation of expression of different subunits of eukaryotic translation elongation factor eEF1 in human glial brain tumors Biopolym. Cell 2008 24, N 4 P. 310–317.
[9] Veremieva M. V., Malysheva T. A., Zozulya Y. P., Rozumenko V. D., Sidorik L. L., Kavsan V. M., Negrutskii B. S., El'skaya A. V. Multisubunit complex eEF1H in human glial tumors: from mRNA to protein Biopolym. Cell 2010 26, N 4 P. 317–321.
[10] Chi K., Jones D. V., Frazier M. L. Expression of an elongation factor 1 gamma-related sequence in adenocarcinomas of the colon Gastroenterology 1992 103, N 1 P. 98–102.
[11] Mimori K., Mori M., Tanaka S., Akiyoshi T., Sugimachi K. The overexpression of elongation factor 1 gamma mRNA in gastric carcinoma Cancer 1995 75, Suppl. 6 P. 1446–1449.
[12] Lew Y., Jones D. V., Mars W. M., Evans D., Byrd D., Frazier M. L. Expression of elongation factor-1 gamma-related sequence in human pancreatic cancer Pancreas 1992 7, N 2 P. 144–152.
[13] Veremieva M., Khoruzhenko A., Zaicev S., Negrutskii B., El'skaya A. Unbalanced expression of the translation complex eEF1 subunits in human cardioesophageal carcinoma Eur. J. Clin. Invest 2011 41, N 3 P. 269–276.
[14] Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: A laboratory manual New York: Cold Spring Harbor Lab. press, 1989.
[15] Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem 1976 72 P. 248–254.
[16] Heng H. H., Bremer S. W., Stevens J. B., Ye K. J., Liu G., Ye C. J. Genetic and epigenetic heterogeneity in cancer: a genome-centric perspective J. Cell Physiol 2009 220, N 3 P. 538–547.
[17] Ishibashi Y., Hanyu N., Nakada K., Suzuki Y., Yamamoto T., Yanaga K., Ohkawa K., Hashimoto N., Nakajima T., Saito H., Matsushima M., Urashima M. Profiling gene expression ratios of paired cancerous and normal tissue predicts relapse of esophageal squamous cell carcinoma Cancer Res 2003 63, N 16. – P. 5159–5164.