Biopolym. Cell. 2010; 26(6):486-491.
http://dx.doi.org/10.7124/bc.000178
Structure and Function of Biopolymers
Expression and subcellular localization of p70S6 kinase under heart failure
1Rozhko O. T., 1Kapustian L. M., 1Bobyk V. I., 1Savynska L. O., 1Tykhonkova I. O., 2Ryabenko D. V., 3Usenko V. S., 1Sidorik L. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Scientific Center "M. D. Strazhesko Institute of Cardiology, MAS of Ukraine"
    5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151
  3. Pathomorphological Laboratory "BIONTEK"
    52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000

Abstract

The PI3K/PDK/Akt/mTOR/p70S6K signaling pathway is primary associated with the activation of insulin receptors and is important for cardiomyocytes survival. p70S6K is a key regulator of the speed and efficiency of protein biosynthesis within the cell. Recently the pro-apoptotic protein BAD has been identified as a new target for p70S6K1. BAD is inactivated in normal cardiomyocytes by p70S6K1 phosphorylation which prevents the cardiomyocytes apoptosis. Aim. To study possible changes in p70S6K1 expression and/or cellular localization at heart failure progression – in DCM-affected human myocardia and murine hearts with experimental DCM-like pathology. Methods. Western-blot analysis and immunohystochemistry. Results. The substantial decrease in p70S6K1 level was observed at the final stage of pathology progression and in the dynamics of DCM pathogenesis as well. For the first time relocalization of the protein to the connective tissue was shown according to the Western-blot results. Conclusions. The data obtained allow us to understand a possible role of p70S6K1 in the regulation of stress-induced apoptotic signaling in cardiomyocytes.
Keywords: р70S6K1, apoptosis, heart failure, Western-blotting, immunohystochemistry
Full text: (PDF, in Russian)

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