Biopolym. Cell. 2010; 26(1):45-50.
Genomics, Transcriptomics and Proteomics
Expression of lectin and defensin genes in Mironovskaya 808 and Roazon wheat cultivars infected with Pseudocercosporella herpotrichoides
1Belava V. N., 2Zeleniy S. B., 1Panyuta O. O., 1Taran N. Yu., 2Pogribniy P. V.
  1. Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022


Aim. The dynamics of expression of protective proteins (lectin and defensin) genes of different genotypes of the winter wheat, affected by P. herpotrichoides, was investigated. Methods. RT-PCR was used for gene expression analysis. Results. We established that the lectin mRNA content in control and infecting plants of the resistant cultivar is considerably higher than in the susceptible one; the character of defensin mRNA accumulation in the investigated cultivars, control and infecting plants, does not change. Conclusion. The spare pool of lectin mRNA in the resistant cultivar is more substantial, than in the susceptible one, that is, possibly, one of the factors of the plant resistance. Inability to support constantly a high level of the protective genes expression seems to influence negatively on the cultivar resistance .
Keywords: lectin, defensin, mRNA, eyespot, wheat


[1] Etzler M. E. Are lectins involved in plant – fungus interactions? Phytopathology 1981 71, N 7 P. 744–746.
[2] Boman H. G. Peptide antibiotics and their role in innate immunity Annu. Rev. Immunol 1995 13 P. 61–92.
[3] Lay F. T., Anderson M. A. Defensins – components of the innate immune system in plants Curr. Protein Pept. Sci 2005 N 6 P. 85–101.
[4] Terras F. R., Schoofs H. M., De Bolle M. F., Van Leuven F., Rees S. B., Vanderleyden J., Cammue B. P., Broekaert W. F. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds J. Biol. Chem 1992 267, N 22 P. 15301–15309.
[5] Butaye K. M., Goderis I. J., Wouters P. F., Pues J. M., Delaure S. L., Broekaert W. F., Depicker A., Cammus B. P., de Bolle M. F. Stable high-level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions Plant J 2004 39, N 3 P. 440–449.
[6] Aerts A. M., Francois I. E., Bammens L., Cammus B. P., Smets B., Winderickx J., Accardo S., De Vos D. E, Thevissen K. The antifungal activity of RsAFP2, a plant defensin from Raphanus sativus, involves the induction of reactive oxygen species in Candida albicans FEBS Lett 2006 580, N 7 P. 1903–1907.
[7] Kovalyova V. A., Gout R. T., Gout I. T. Production of Scots pine recombinant 1 and its antifungal activity. Biopolym. cell 2008 24, N 5 P. 377–384.
[8] Kovalyova V. A., Gout I. T., Gout R. T. Characterization of defensin-like proteims from Scots pine seedlings. Biopolym. cell 2006 22, N 2 P. 126–131.
[9] Terras F. R., Eggermont K., Kovaleva V. Raikhel N. V., Osborn R. W., Kester A., Rees S. B., Torrekens S., van Leuven F., Vanderleyden J., Cammue B. P. A., Broekaert W. F. Small cysteine-rich antifungal proteins from radish: their role in host defense Plant Cell 1995 7, N 5 P. 573–588.
[10] Tarchevsky I. A. Pathogen-Induced Plant Proteins Appl. Biochem. and Microbiol 2001 37, N 5 P. 441–455.
[11] Belava V., Panyuta o., Taran N. Model system of infection and level of winter wheat (Triticum aestivum L.) resistance estimation to eyespot agent (Pseudocercosporella herpotrichoides (Fron) Deighton). Karantyn i Zakhyst Roslyn 2008; N 7 P. 25–28.
[12] Wallwork H., Spooner B. Tapesia yallundae – the teleomorph of Pseudocercosporella herpotrichoides Transact. Brit. Mycol. Soc 1988 91, N 44;703–705.
[13] Bartlett J., Stirling D. PCR Protocols, 2nd ed Totowa: Humana Press, 2003 556 p.
[14] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual New York: Cold Spring Harbor Lab. press, 1982 545 p.
[15] Lepekhin E. A., Yalovoi A. I., Rybak V. I. Activity and specificity carbohydrates in lectins of germinating maize grains Russ. J. Plant Physiol 1986 33, N 2 P. 390–394.
[16] Peumans W. J., Stinissen H. M., Carlier A. A genetic basis for the origin of six different isolectins in hexaploid wheat. Planta 1982 154, N 6 P. 562–567.
[17] Shakirova F. M. Nonspecific resistance to stressful factors and its regulation Ufa: Gilem, 2001 160 p.
[18] Yamamoto H., Tani T., Naito N. Changes in protein contents of oat leaves during the resistant reaction against Puccinia coronata avenae. Phytopathology 1975 82, N 2 P. 138–145.
[19] Medvedeva T. E., Chalenko G. I., Vasyukova N. I., Ozeretskovskaya O. L. Influence of suppressor of the potato late blight causal agent on wounding reparation of potato tubers. Dokl Akad Nauk SSSR. 1985; 280(3):764–767.