Biopolym. Cell. 2009; 25(6):509-512.
http://dx.doi.org/10.7124/bc.0007FF
Short Communications
Expression of nicotinic acetylcholine receptors on human B-lymphoma cells
1Omelchenko D. M., 1Skok M. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

Aim. To find a correlation between the level of nicotinic acetylcholine receptor (nAChR) expression and B lymphocyte differentiation or activation state. Methods. Expression of nAChRs in the REH, Ramos and Daudi cell lines was studied by flow cytometry using nAChR subunit-specific antibodies; cell proliferation was studied by MTT test. Results. It is shown that the level of α4β2β4 and α7 nAChRs expression increased along with B lymphocyte differentiation (Ramos > REH) and activation (Daudi > Ramos) and depended on the antigen-specific receptor expression. The nAChR stimulation/blockade did not influence the intensity of cell proliferation.
Keywords: nicotinic acetylcholine receptor, B- lymphocytes
Full text: (PDF, in English) (PDF, in Ukrainian)

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