Biopolym. Cell. 2009; 25(6):500-503.
Short Communications
The chitinase 3-like human protein 2 (CHI3L2) activates ERK1/2 signaling pathway in human embryonic kidney cells (HEK293)
1Areshkov P. O., 1Kavsan V. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Aim. To show that CHI3L2 activates the extracellular signal regulated kinase (ERK1/ERK2) mediated cascade similarly to the homologous CHI3L1 protein. Methods. The involvement of CHI3L2 in ERK1/ERK2 phosphorylation was evaluated by Western blotting with either polyclonal anti-ERK1/ERK2 or monoclonal anti-phospho-ERK1/ERK2 antibodies. Results. The checking of CHI3L2 possibility to initiate MAPK cascade showed the phosphorylation of ERK1/ERK2 in HEK293 after addition of CHI3L2 to DMEM. Conclusions. Chitinase-like protein CHI3L2 activates phosphorylation of ERK1/ERK2 in human embryonic kidney cells (HEK293) and thus initiates the MAP kinase signaling cascade which may lead to increased cell proliferation or differentiation.
Keywords: chitinase 3-like protein 1 (CHI3L1, HC gp-39, YKL- 40), chitinase 3-like protein 2 (CHI3L2, YKL-39), MAP kinase, ERK1/ERK2


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