Biopolym. Cell. 2009; 25(4):279-289.
Molecular and Cell Biotechnologies
The procedure of ethanol determination in wine by enzyme amperometric biosensor
1, 2Goriushkina T. B., 1, 2Orlova A. P., 3Veryk G. M., 1Soldatkin A. P., 1Dzyadevych S. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601
  3. National Institute for Vine and Wine "Magarach"
    31, Kirov Str., Yalta, Ukraine, 98600


Aim. Development of the procedure of ethanol determination in wine by an enzyme amperometric biosensor. Methods. The amperometric biosensor method of ethanol analysis has been used in this work. Results. The paper presents comparative analysis of two methods of alcohol oxidase (AO) immobilization for development of amperometric biosensor for ethanol determination in wine. The method of AO immobilization in glutaraldehyde vapour was chosen as optimal for this purpose. The selectivity, operational and storage stability, and pH-optimum for operation of the created biosensor were determined. The procedure of ethanol determination in wine by amperometric biosensor on the basis of platinum printed electrode SensLab and AO was optimized. The analysis of ethanol concentration in wine and must samples was carried out using the developed high-stable biosensor. A good correlation between the data obtained by the biosensor and densitometry methods was shown. Conclusion. The proposed method of ethanol analysis could be used in wine production.
Keywords: amperometric biosensor, alcohol oxidase, ethanol, wine, mus


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