Biopolym. Cell. 2009; 25(2):120-125.
http://dx.doi.org/10.7124/bc.0007D4
Molecular and Cell Biotechnologies
Agrobacterium-mediated transformation of Cichorium intybus L. with interferon-α2b gene
1Matvieieva N. A., 1Shachovsky A. M., 1Gerasymenko I. M., 1Kvasko O. Yu., 1Kuchuk N. V.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

An efficient method for the plant regeneration and Agrobacterium mediated transformation with interferon-α2b gene has been developed for chicory C. intybus L. cv. Pala rossa. The regeneration with efficiency about 100 % was induced on the MS medium supplemented with 0.5–2.5 mg/l kinetin and 0.5 mg/l NAA. The transformed plantlets were recovered at a frequency 26,9 % on basal medium with 25 mg/l kanamycin. According to PCR-analysis the nptII and ifn-α2b genes were integrated into the genome of transformed plants.
Keywords: transformation, Agrobacterium, Cichorium intybus, interferon
Full text: (PDF, in English) (PDF, in Ukrainian)

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