Biopolym. Cell. 2008; 24(6):508-512.
Short Communications
Efficient gene delivery into mammalian cells by baculovirus vector in vitro
1Vagyna I. N., 1Anopriyenko O. V., 1Zaharuk O. A., 2Gorchev V. F., 1Strokovska L. I., 1Solomko A. P.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 - Palladin Institute of Biochemistry, NAS of Ukraine
9, Leontovycha Str., Kyiv, Ukraine, 01601
Abstract
The recombinant baculovirus vector with EGFP reporter gene under the control of strong CAG promoter cassette was used for transduction of normal (HEK293) and tumor (HeLa) human cell lines. Dependence of transduction efficiency on the virus dose, time of virus incubation, temperature and cell type is shown. Transient reporter gene expression gradually diminished and was detected in single cells on the 15th day after transduction.
Keywords: recombinant baculovirus, AcMNPV, transduction, transient expression, gene therapy
Full text: (PDF, in English) (PDF, in Russian)
References
[1]
Hofmann C., Sandig V., Jennings G., Rudolph M., Schlag P., Strauss M. Efficient gene transfer into human hepatocytes by baculovirus vectors Proc. Natl Acad. Sci. USA 1995. 92, N 22:10099–10103.
[2]
Shoji I., Aizaki H., Tani H., Ishii K., Chiba T., Saito I., Miyamura T., Matsuura Y. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J. Gen. Virol. 1997; 78(10):2657–2664.
[3]
Tani H., Limn C. K., Yap C. C., Onishi M., Nozaki M., Nishimune Y., Okahashi N., Kitagawa Y., Watanabe R., Mochizuki R., Moriishi K., Matsuura Y. In vitro and in vivo gene delivery by recombinant baculoviruses J. Virol 2003 77, N 18:9799–9808.
[4]
Kenoutis C., Efrose R. C., Swevers L., Lavdas A. A., Gaitanou M., Matsas R., Iatrou K. Baculovirus-mediated gene delivery into mammalian cells does not alter their transcriptional and differentiating potential but is accompanied by early viral gene expression J. Virol. 2006 80, N 8:4135–4146.
[5]
Hu Y.-C. Baculovirus vectors for gene therapy Insect viruses: biotechnological applications. Ed. D. C. Bonning New York: Elsevier, 2006 Vol. 68:287–320.
[6]
Condreay J. P., Witherspoon S. M., Clay W. C., Kost T. A. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector Proc. Nat. Acad. Sci. USA 1999 96, N 1:127–132.
[7]
Fujita R., Matsuyama T., Yamagishi J., Sahara K., Asano S., Bando H. Expression of Autographa californica multiple nucleopolyhedrovirus genes in mammalian cells and upregulation of the host beta-actin gene J. Virol 2006 80, N 5:2390–2395.
[8]
Boyce F. M., Bucher N. L. Baculovirus-mediated gene transfer into mammalian cells Proc. Nat. Acad. Sci. USA 1996 93, N 6:2348–2352.
[9]
King L. A., Possee R. D. The baculovirus expression system. A laboratory guide. London: Chapmann and Hall, 1992 220 p.
[10]
Lakin GF. Biometrics. Vysshaya shkola, M. 1990. 352 p.
[11]
Cheng T., Xu C. Y., Wang Y. B., Chen M., Wu. T., Zhang J., Xia N. S. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J. Gastroenterol. 2004; 10(11):1612–1618.
[12]
Hsu C. S., Ho Y. C., Wang K. C., Hu Y. C. Investigation of optimal transduction conditions for baculovirus-mediated gene delivery into mammalian cells Biotechnol. Bioeng 2004 88, N 1:42–51.
[13]
Kost T. A., Condreay J. P., Jarvis D. L. Baculovirus as versatile vectors for protein expression in insect and mammalian cells Nat. Biotechnol 2005 23, N 5:567–575.
