Biopolym. Cell. 2008; 24(3):254-259.
Phosphatidylcholine and phosphatidylinositol are allosteric regulators of 5-lipoxygenase from potato tubers
- Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
1, Murmans'ka Str., Kyiv, Ukraine, 02094
Kinetic constants of the linoleic acid oxidation catalized by purified 5-lipoxygenase from Solanum tuberosum tubers have been determined. Hydrophilic and hydrophobic forms of the enzyme have been shown to bind linoleic acid with positive cooperativity in micellar system. Phosphatidylcholine and phosphatidylinositol binding to lipoxygenase results in the release of the substrate from the enzyme, causing the decrease or increase of the reaction rate, respectively. Presented findings imply the allosteric regulation of the 5-lipoxygenase activity by these phospholipids.
Keywords: 5-lipoxygenase, phospholipids, allosteric regulation
 Liavonchanka A, Feussner I. Lipoxygenases: occurrence, functions and catalysis. J Plant Physiol. 2006;163(3):348-57.
 Walther M, Wiesner R, Kuhn H. Investigations into calcium-dependent membrane association of 15-lipoxygenase-1. Mechanistic roles of surface-exposed hydrophobic amino acids and calcium. J Biol Chem. 2004;279(5):3717-25.
 Kulkarni S, Das S, Funk CD, Murray D, Cho W. Molecular basis of the specific subcellular localization of the C2-like domain of 5-lipoxygenase. J Biol Chem. 2002;277(15):13167-74.
 Butovich IA, Parshikova TV, Babenko VM. et al. Regulatory role of phospholipids in the oxidation of linoleic acid by 5-lipoxygenase. Biol. Membrane. 1992; 9(6):611вЂ“6.
 Butovich IA, Babenko VM, Livarchuk LV, Mogilevich TV, Kukhar VP. Activation of linoleic acid oxidation by potato 5-lipoxygenase indused by phosphatidic acid. Biokhimiia. 1991; 56(6):1077-81.
 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
 Ornstein M. Disk electrophoresis-I Background and Theory. Ann N Y Acad Sci. 1964;121:321-49.
 Gibian MJ, Vandenberg P. Product yield in oxygenation of linoleate by soybean lipoxygenase: the value of the molar extinction coefficient in the spectrophotometric assay. Anal Biochem. 1987;163(2):343-9.
 Gordon J. Organic Chemistry of Electrolyte Solutions. New York: Wiley, 1979. 712.
 Kurganov BI. Allosteric enzymes. Moscow: Nauka, 1978. 247 p.
 Aharony D, Stein RL. Kinetic mechanism of guinea pig neutrophil 5-lipoxygenase. J Biol Chem. 1986;261(25):11512-9.
 Mogul R, Johansen E, Holman TR. Oleyl sulfate reveals allosteric inhibition of soybean lipoxygenase-1 and human 15-lipoxygenase. Biochemistry. 2000;39(16):4801-7.
 Hornig C1, Albert D, Fischer L, H?rnig M, Radmark O, Steinhilber D, Werz O. 1-Oleoyl-2-acetylglycerol stimulates 5-lipoxygenase activity via a putative (phospho)lipid binding site within the N-terminal C2-like domain. J Biol Chem. 2005;280(29):26913-21.
 Butovich I. A., Soloshonok V. A., Solodenko V. A., Kukhar V. P. Activation of 5-lipoxygenase by lipophylic n-alkyl-containing acids is an allosteric process. Russian Journal of Bioorganic Chemistry 1990, 16 (2):270-271
 Kharchenko OV, Kulinichenko HI, Butovych IA. Kinetic mechanisms of linoleic acid oxidation by 5-lipoxygenase from Solanum tuberosum L. Ukr Biokhim Zh. 1999;71(4):40-4.
 Butovich IA, Kukhar' VP. Amphiphilic aliphatic acids--activators of 5-lipoxygenase. The adsorption-micellar regulatory mechanism. Dokl Akad Nauk SSSR. 1991;316(6):1486-90.
 Butovich IA, Kharchenko OV, Paboka IuN, Kazachkov MG. Characteristics of the aggregative state of the substrate in the reaction of 5-lipoxygenase oxidation of linoleic acid. Ukr Biokhim Zh. 2001;73(2):39-43.
 Butovich IA, Luk'yanova SM, Reddy CC. Oxidation of linoleyl alcohol by potato tuber lipoxygenase: kinetics and positional, stereo, and geometrical (cis, trans) specificity of the reaction. Arch Biochem Biophys. 2000;378(1):65-77.
 Butovich IA, Reddy CC. Enzyme-catalyzed and enzyme-triggered pathways in dioxygenation of 1-monolinoleoyl-rac-glycerol by potato tuber lipoxygenase. Biochim Biophys Acta. 2001;1546(2):379-98.