Biopolym. Cell. 2008; 24(3):238-245.
http://dx.doi.org/10.7124/bc.0007A6
Molecular Biomedicine
Changes in the content of molecular chaperone Hsp60 in heart tissue at dilated cardiomyopathy
1Kapustian L. M., 1Rozhko O. T., 1Bobyk V. I., 1Kroupska I. V., 2Riabenko D. V., 3Khozhaenko Yu. S., 3Gurtovyy V. A., 3Usenko V. S., 1Sidorik L. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Scientific Center "M. D. Strazhesko Institute of Cardiology, MAS of Ukraine"
    5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151
  3. Pathomorphological Laboratory "BIONTEK"
    52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000

Abstract

The changes of Hsp60 content in heart tissue at dilated cardiomyopathy (DCM) were investigated. An increase in Hsp60 level was observed in human hearts affected by DCM as well as in the hearts of mice with disease model similar to human DCM. We observed the increase in Hsp60 level in mitochondria and decrease in cytoplasmic fraction, which could be one of the reasons for apoptotic responce in cardiomyocytes at heart failure associated with chronic stress. The results of immunohystochemical analysis of mice hearts with the model human-like pathology demonstrate heterogeneity in chaperone levels. A considerable increase in Hsp60 staining was observed only in some cardiomyocytes, possibly in those with activated antistress mechanisms of cell defense against chronic stress.
Keywords: molecular chaperone, Hsp60, dilated cardiomyopathy, cardiomyocytes, apoptosis
Full text: (PDF, in Ukrainian)

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