Biopolym. Cell. 2008; 24(3):225-230.
Cell Biology
Application of carbocyanine fluorescent probes for evaluation of the functional state of cell cultures after cryoconservation
1Goncharuk E. I., 1Onischenko E. V., 1Timon V. V., 1Petrenko T. F., 2Borovoy I. A., 2Maliukin Yu. V., 1Grishchenko V. I.
  1. Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
    23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
  2. State Scientific Institution "Institute for Single Crystals" NAS of Ukraine
    60, Lenin Ave., Kharkiv, Ukraine, 61001


Application of C2, C9 and JC-1 dyes for characterizing human fibroblast diploid cell line after cryoconservation was studied. It was shown that these probes might be used to monitor the functional state of cryocenserved cells.
Keywords: fluorescence, probes, human fibroblasts, cell culture, cryoconservation


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