Biopolym. Cell. 2008; 24(3):218-224.
Structure and Function of Biopolymers
Identification of phosphate transporter NaPi2b as MX35 cancer antigen by modified SEREX approach
1Kiyamova R. G., 1Gryshkova V. S., 2Usenko V. S., 2Khozaenko Y. S., 2Gurtovyy V. A., 3Yin B., 3Ritter G., 3Old L., 4Gout I. T., 1Filonenko V. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Pathomorphological Laboratory "BIONTEK"
    52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000
  3. Ludwig Institute for Cancer Research, New-York branch at Memorial Sloan-Kettering Cancer Center
    1275 York Avenue, BOX 32 New York, NY 10021-6007, USA
  4. Department of Biochemistry and Molecular Biology,
    University College London
    Gower Str., London WC1E 6BT, UK


In this study we describe the identification of sodium-dependent phosphate transporter NaPi2b as MX35 cancer-associated antigen. To achieve this goal we have screened extensively a cDNA expressing library from ovarian cancer cell line OVCAR3 with monoclonal antibody MX35. To further confirm the authenticity of this finding, we showed that bacterially and baculovirally expressed NaPi2b is specifically recognized by MX35 antibody. Moreover, the validity of these results was verified in a parallel study involving affinity purification and mass spectrometry. The epitope for MX35 monoclonal antibody was mapped to the largest extracellular loop of NaPi2b. Taken together, this study uncovers the identity of MX35 antigen and provides molecular tools for studying its function in normal and cancer tissues.
Keywords: MX35 monoclonal antibodies, ovarian cancer, sodium-dependent phosphate cotransporter NaPi2b


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