Biopolym. Cell. 2008; 24(2):129-134.
http://dx.doi.org/10.7124/bc.00079A
Genome and Its Regulation
PCR-marking of genes of Cbf-family, controlling low temperature acclimatization of barley and soft wheat
1Balashova I. A., 1Balvinska M. S., 2Fait V. I., 1Galaeva M. V., 1Sivolap Yu. M.
  1. South Biotechnological Center of Plant Cultivation
    Ovidiopol'skaya dor., 3, Odessa, Ukraine, 65036
  2. Plant breeding and Genetics Institute, National Centre of Seed and Cultivar Investigation
    Ovidiopol'skaya dor., 3, Odessa, Ukraine, 65036

Abstract

Polymorphism in the genotypes of barley and soft wheat, contrast in high and low temperature tolerance, was detected by STS-PCR-analysis with primers, directed to Cbf2 gene. It is supposed that polymorphic DNA-loci are associated with the presence of Cbf2 gene in different allelic state in genotypes with high and low cold stress tolerance.
Keywords: barley, wheat, frost resistance, Cbf-genes, STS-PCR
Full text: (PDF, in Russian)

References

[1] Levitt J. Responses of plants to environmental stresses. New York: Acad. press, 1980. Vol. 1. 697 p.
[2] Xin Z, Browse J. Cold comfort farm: the acclimation of plants to freezing temperatures. Plant Cell Environ. 2000; 23(9):893–902.
[3] Thomashow MF. Role of cold-responsive genes in plant freezing tolerance. Plant Physiol. 1998;118(1):1-8.
[4] Xiao FH, Xue GP. Analysis of promoter activity of late embryogenesis abundant protein genes in barley seedlings under conditions of water deficit. Plant Cell Report. 2001; 20(7):667–673.
[5] Murelli C, Rizza F, Albini FM, Dulio A, Terzi V, Cattivelli L. Metabolic changes associated with cold-acclimation in contrasting cultivars of barley. Physiol. Plant. 1995; 94(1):87–93.
[6] Uemura M, Joseph RA, Steponkus PL. Cold Acclimation of Arabidopsis thaliana (Effect on Plasma Membrane Lipid Composition and Freeze-Induced Lesions). Plant Physiol. 1995;109(1):15-30.
[7] Gilmour SJ, Fowler SG, Thomashow MF. Arabidopsis transcriptional activators CBF1, CBF2, and CBF3 have matching functional activities. Plant Mol Biol. 2004;54(5):767-81.
[8] Xue GP. Characterisation of the DNA-binding profile of barley HvCBF1 using an enzymatic method for rapid, quantitative and high-throughput analysis of the DNA-binding activity. Nucleic Acids Res. 2002;30(15):e77.
[9] Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J. 2003;33(4):751-63.
[10] Kobayashi F, Takumi S, Kume S, Ishibashi M, Ohno R, Murai K, Nakamura C. Regulation by Vrn-1/Fr-1 chromosomal intervals of CBF-mediated Cor/Lea gene expression and freezing tolerance in common wheat. J Exp Bot. 2005;56(413):887-95.
[11] Skinner JS, von Zitzewitz J, Szucs P, Marquez-Cedillo L, Filichkin T, Amundsen K, Stockinger EJ, Thomashow MF, Chen TH, Hayes PM. Structural, functional, and phylogenetic characterization of a large CBF gene family in barley. Plant Mol Biol. 2005;59(4):533-51.
[12] Sivolap Yu. M., Kalendar' R. N., Netsvetaev V. P. Use of Polymerase Chain Reaction Products to Map the Barley (Hordeum vulgare L.) Genome.). Russian Journal of Genetics 1997; 33(1):43-49.
[13] Sivolap IuM, Kalendar' RN, Chebotar' SV. The genetic polymorphism of cereals demonstrated by PCR with random primers. Tsitol Genet. 1994;28(6):54-61.
[14] Xue GP. The DNA-binding activity of an AP2 transcriptional activator HvCBF2 involved in regulation of low-temperature responsive genes in barley is modulated by temperature. Plant J. 2003;33(2):373-83.
[15] Trofimovskaya A. YA. Yachmen'. Leningrad.: Kolos, 1972. 294 p.
[16] Litvinenko MA, Lifenko SP, Druzyak VV, Druzyak VG. Effect of sowing time and sublethal temperatures on winter survival and yield of winter wheat. Visn. agrar. nauk. 2004. 5:27-32.
[17] Morgun VV, Logvinenko VF, Ulich LI, Kravets VS. Winter-hardiness and frost- resistance of modern winter wheat cultivars. Fiziologiia i biokhimiia kul'turnykh rasteniy. 2000; 32(4):255-60.
[18] Pilipenko MV, Chebotar SV, Fayt VI, Sivolap YuM. Microsatellite marker analysis of winter hardness in wheat. Eur Wheat Neuploid Co-operative Newsletter. Amsterdam, 2006:117–120.