Biopolym. Cell. 2007; 23(5):410-415.
Cell Biology
Induction of gene mutations by lectins of different origin and cytokine EMAP II in somatic mammalian cells in vitro
1Kovalenko O. O., 1Lukash L. L., 1Lukash S. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Exogenous carbohydrate binding proteins, namely, three lectins of different origin and cytokine EMAPII, have revealed the ability to induce mutations in hprt locus in Chinese hamster cells in vitro. In the investigations of their biological action at the wide range of doses (from 0.002 to 2000 µg/ml) direct dependence of mutagenic effect on the concentration of foreign protein in cellular system has been manifested. Mathematical modelling has shown that resulting curve consists of three processes: the first one proceeds with saturation, the second one – with linear dependence on the dose, and the third one is described by non-linear equation of the second degree. Mutagenic activity of lectins is realized according to the latter equation and characterized by gradual increasing of mutation frequency with the increase in the protein dose.
Keywords: mammalian cells, cytokine, lectin, resistance, mutations, hprt locus, apoptosis, mathematical modelling

References

[1] Loris R. Principles of structures of animal and plant lectins. Biochim Biophys Acta. 2002;1572(2-3):198-208.
[2] Lis H, Sharon N. Lectins: Carbohydrate-Specific Proteins That Mediate Cellular Recognition. Chem Rev. 1998;98(2):637-674.
[3] Boettner DR, Huston C, Petri WA. Galactose/N-acetylgalactosamine lectin: The coordinator of host cell killing. J Biosciences. 2002;27(6):553–7.
[4] Lukash LL, Karpova IS, Miroshnichenko OS, Tikhonova TN, Lylo VV, Man'ko VG, Sukhorada EM, Golynskaia EL. [The effect of the lectin from Sambucus nigra inflorescences on spontaneous and alkylating agent-induced mutagenesis in mammalian somatic cells]. Tsitol Genet. 1997;31(5):52-60. Russian.
[5] Macewicz LL, Kovalenko OA, Sukhorada OM, Lukash LL. Investigation of the influence of proteins on the genetic variability of cell populations in vitro. Factors Experimental evolution of organisms: Coll. Science, Labor. Ed. MV Royik. Kyiv, Agricultural Science, 2003:91-7.
[6] Macewicz LL, Lukash LL. Influence of some low molecular weight compounds and Sambucus nigra bark lectin modifying action on variability in populations of cells in vitro. Factors Experimental evolution of organisms: Coll. Science, Labor. Ed. MV Royik. Kyiv, Agricultural Science, 2004; 111-6.
[7] Karpova IS, Koretskaya NV. Study on modifying action of lectins on the toxic and mutagenic effects of Ni(II) ions in Bacillus subtills culture. Biopolym Cell. 2003; 19(3):224-30.
[8] Kao J, Houck K, Fan Y, Haehnel I, Libutti SK, Kayton ML, Grikscheit T, Chabot J, Nowygrod R, Greenberg S, et al. Characterization of a novel tumor-derived cytokine. Endothelial-monocyte activating polypeptide II. J Biol Chem. 1994;269(40):25106-19.
[9] Berger AC, Alexander HR, Tang G, Wu PS, Hewitt SM, Turner E, Kruger E, Figg WD, Grove A, Kohn E, Stern D, Libutti SK. Endothelial monocyte activating polypeptide II induces endothelial cell apoptosis and may inhibit tumor angiogenesis. Microvasc Res. 2000;60(1):70-80.
[10] Dubrovsky AL, Brown Jn, Kornelyuk AI, Murray JC, Matsuka GKh. Bacterial expression of full-length and truncated forms of cytokine EMAP-2 and cytokine-like domain of mammalian tyrosyl-tRNA synthetase. Biopolym Cell. 2000; 16(3):229-35.
[11] Lukash LL. The regulation of a variability of somatic mammalian cell genome under the influence of exogeneous biological factors. Biopolym Cell. 2004; 20(1-2):93-105.
[12] Lakhtin VM. [Molecular organization of lectins]. Mol Biol (Mosk). 1994;28(2):245-73.
[13] Barbieri L, Ciani M, Girb?s T, Liu WY, Van Damme EJ, Peumans WJ, Stirpe F. Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins. FEBS Lett. 2004;563(1-3):219-22.
[14] Buzhievskaya TI, Lukash LL, Podolsky Eksperimental model for the study of mutagenesis and transformation induced by viruses and nucleic acids. Molecular biology techniques. Naukova Dumka, 1986:147-58.
[15] Lukash LL, Podolskaya SV, Suhorada HM, Kostetskaya KV, Kostetsky IE, Varzanova IS, Patskovsky YuV, Vavilina IV, Deis SV. The influence of alkylating agent MNNG on mutagenic effect of exogeneous recombinant DNA. Biopolym. Cell. 1995; 11(1):87-91.
[16] Moiseenko EV, Luss EV, Petrova ON, Shapiro NI. Studies of hypoxanyhineguanine phosphoribosyltransferase (HGPRT) mutans exhibiting anomalous behavior under selective conditions I. General characteristics of two mutant chinese hamster cell clones. Genetika. 1978, 14(5):829-36.
[17] Kovalenko OO, Kostetskaia KV, Lukash LL. The influence of lectins of different origin on mutagenic process of mammalian somatic cells in vitro. Biopolym Cell. 2006; 22(1):33-7.
[18] Peumans WJ, Hao Q, Van Damme EJ. Ribosome-inactivating proteins from plants: more than RNA N-glycosidases? FASEB J. 2001;15(9):1493-506.
[19] Durnev AD, Seredenin SB. Mutagens. Screening and prevention of pharmacological effects. M.: Meditsina, 1998. 365 p.