Biopolym. Cell. 2007; 23(5):405-409.
http://dx.doi.org/10.7124/bc.00077A
Structure and Function of Biopolymers
Protein-lipid and protein-protein interactions of Bcr PH domain
1Miroshnychenko D. O., 1Dubrovska A. M., 1Telegeev G. D., 1Maliuta S. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Bcr is a partner of Abl in reciprocal translocation t(9;22) which leads to Philadelphia chromosome formation. In the present study we analyzed Bcr PH domain functions and determined lipid specificity of PH domain in vitro. The technical approach for determining PH domain protein-protein interactions using mass-spectrometry was developed. Interactions with SMC1, β-tubulin, zizimin1 and PLCε were confirmed in vitro and in vivo.
Keywords: Bcr-Abl, PH domain, mass spectrometry
Full text: (PDF, in English) (PDF, in Ukrainian)

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