Biopolym. Cell. 2007; 23(4):301-306.
Structure and Function of Biopolymers
Investigation of structure-function interaction of polyhedron of Bombyx mori Nuclear Polyhedrosis Virus (NPV) with polyhedra protease and RNA. 3. Polyhedras contain small RNA
1Kozlov E. A., 2Vudmaska M. I., 1Bobrovskaja M. T., 1Shirina T. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. The immunobiotechnological Scientific Industrial Enterprise, Joint-Stock Company "Diaproph-Med"
    35, Svetlitsky str., Kyiv, Ukraine, 04123


It was shown that polyhedras of Bombyx mori Nuclear Polyhedrosis Virus contain RNA with molecular weight of 17-18 kDa (50-60 nucleotides). This RNA is likely to belong to the minor RNA class and its origin is discussed.
Keywords: nuclear polyhedrosis virus, polyhedra RNA, microRNA


[1] Kozlov EA, Soguliaeva VM, Levitina TL, Vereshchak V, Serebriany? SV. [Purification of the polyhedral protein of nuclear polyhedrosis virus of mulberry silkworm and association of the protein in solution]. Biokhimiia. 1969;34(4):679-85.
[2] Ruvkun G. Molecular biology. Glimpses of a tiny RNA world. Science. 2001;294(5543):797-9.
[3] Zamore PD. Ancient pathways programmed by small RNAs. Science. 2002;296(5571):1265-9.
[4] Kr?tzfeldt J, Poy MN, Stoffel M. Strategies to determine the biological function of microRNAs. Nat Genet. 2006;38 Suppl:S14-9.
[5] Cullen BR. Viruses and microRNAs. Nat Genet. 2006;38 Suppl:S25-30.
[6] Kozlov EA, Nijazova N, Bobrovskaja MT, Shirina TV. Investigation of structure-function interaction of Bombyx mori nuclear polyhedrosis virus polyhedrin with polyhedra protease and RNA. Effect of protease on polyhedrin dissociation during Sepharose 6B gel-filtration. Biopolym Cell. 2006; 22(3):192-200.
[7] Hamilton A, Voinnet O, Chappell L, Baulcombe D. Two classes of short interfering RNA in RNA silencing. EMBO J. 2002;21(17):4671-9.
[8] Maniatis T, Fritsch E, Sambrook J. Molecular cloning. A laboratory manual. New York: Cold Spring Harbor Lab. press, 1982. 545 p.
[9] Mette MF, Aufsatz W, van der Winden J, Matzke MA, Matzke AJ. Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J. 2000;19(19):5194-201.
[10] Kozlov EA, Levitina TL, Gusak NM. The primary structure of baculovirus inclusion body proteins. Evolution and structure-function aspects. Curr Top Microbiol Immunol. 1986;131:135-64.
[11] Raftopoulou M. microRNA signals cell fate. Nat Cell Biol. 2006;8(2):112.
[12] Boehm M, Slack FJ. MicroRNA control of lifespan and metabolism. Cell Cycle. 2006;5(8):837-40.
[13] Couzin J. Cancer biology. A new cancer player takes the stage. Science. 2005;310(5749):766-7.
[14] Ruvkun G. Clarifications on miRNA and cancer. Science. 2006;311(5757):36-7.
[15] Jover-Gil S, Candela H, Ponce MR. Plant microRNAs and development. Int J Dev Biol. 2005;49(5-6):733-44.
[16] Plasterk RH. Micro RNAs in animal development. Cell. 2006;124(5):877-81.
[17] Seggerson K, Tang L, Moss EG. Two genetic circuits repress the Caenorhabditis elegans heterochronic gene lin-28 after translation initiation. Dev Biol. 2002;243(2):215-25.
[18] Sempere LF, Sokol NS, Dubrovsky EB, Berger EM, Ambros V. Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and broad-Complex gene activity. Dev Biol. 2003;259(1):9-18.
[19] Bashirullah A, Pasquinelli AE, Kiger AA, Perrimon N, Ruvkun G, Thummel CS. Coordinate regulation of small temporal RNAs at the onset of Drosophila metamorphosis. Dev Biol. 2003;259(1):1-8.
[20] Carrington JC, Ambros V. Role of microRNAs in plant and animal development. Science. 2003;301(5631):336-8.
[21] Lee Y, Jeon K, Lee JT, Kim S, Kim VN. MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 2002;21(17):4663-70.
[22] Zeng Y, Yi R, Cullen BR. Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. EMBO J. 2005;24(1):138-48.
[23] Cai X, Hagedorn CH, Cullen BR. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA. 2004;10(12):1957-66.
[24] Okano K, Vanarsdall AL, Mikhailov VS, Rohrmann GF. Conserved molecular systems of the Baculoviridae. Virology. 2006;344(1):77-87.
[25] Berezikov E, Cuppen E, Plasterk RHA. Approaches to microRNA discovery. Nat Genet. 2006;38(6s):S2–S7.