Biopolym. Cell. 2007; 23(3):250-254.
The expression of O6-methylguanine-DNA methyltransferase in the spontaneously immortalized mouse cell line G1 and its sublines G1-OA and G1-T
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The expression of Mgmt gene was investigated by Western blot analysis in the spontaneously immortalized mouse cell line G1 and in its sublines G1-OA and G1-T at different passages of in vitro cultivation. The highest level of Mgmt expression has been revealed in G1-T subline cells and the lowest вЂ“ in G1-OA subline cells. The increase in the level of DNA repair enzyme MGMT was observed in the cells of mouse cell line G1 as well as in its subline G1-OA at later passages of in vitro cultivation. Since the G1-OA subline is characterized by the highest frequencies of chromosomal aberrations, micronucleate and multinucleate cells, it is possible to suppose a role of deficiency of DNA repair enzyme MGMT in the increase of the chromosomal aberration level in G1-OA subline.
Keywords: mammalian cells in vitro, O6-methylguanine-DNA methyltransferase (MGMT), chromosomal aberrations
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