Modification of immune SPR biosensor surface at nonylphenol analysis

Demchenko, A. V.; Starodub, M. F.

doi:10.7124/bc.000761

Biopolym. Cell. 2007; 23(2):143-147.

http://dx.doi.org/10.7124/bc.000761

Bioorganic Chemistry

Modification of immune SPR biosensor surface at nonylphenol analysis

1Demchenko A. V., 1Starodub M. F.

Palladin Institute of Biochemistry, NAS of Ukraine
9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

The influence of the state of transducer surface of immune SPR biosensor on the sensitivity and stability of its «direct» detection of nonylphenol in solutions has been investigated. The biosensor surface was modified with polyelectrolytes, dodecanethiol, and dextran sulfate and glutaraldehyde. It was shown that the highest sensitivity (up to 10 ng/ml) and stability of the biosensor response were achieved at the surface modification with dodecanthiol and simultaneous creation of intermediate layer with protein A Staphylococcus aureus for the orientation of active sites of specific antibodies toward solution.

Keywords: nonylphenol, immune biosensor, polyelectrolytes, dodecanethiol, dextran sulfate, glutaraldehyde

Full text: (PDF, in English) (PDF, in Ukrainian)

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