Biopolym. Cell. 2007; 23(2):143-147.
Bioorganic Chemistry
Modification of immune SPR biosensor surface at nonylphenol
analysis
1Demchenko A. V., 1Starodub M. F.
- Palladin Institute of Biochemistry, NAS of Ukraine
9, Leontovycha Str., Kyiv, Ukraine, 01601
Abstract
The influence of the state of transducer surface of immune SPR
biosensor on the sensitivity and stability of its «direct» detection of
nonylphenol in solutions has been investigated. The biosensor
surface was modified with polyelectrolytes, dodecanethiol, and
dextran sulfate and glutaraldehyde. It was shown that the highest
sensitivity (up to 10 ng/ml) and stability of the biosensor response
were achieved at the surface modification with dodecanthiol and
simultaneous creation of intermediate layer with protein A Staphylococcus aureus for the orientation of active sites of specific
antibodies toward solution.
Keywords: nonylphenol, immune biosensor, polyelectrolytes, dodecanethiol, dextran sulfate, glutaraldehyde
Full text: (PDF, in English) (PDF, in Ukrainian)
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