Biopolym. Cell. 2007; 23(2):93-99.
Molecular and Cell Biotechnologies
The development of DNA-vaccine against classical swine fever
1Pokholenko I. A., 1Ruban T. A., 1Sukhorada O. M., 2Deriabin O. M., 1Tytok T. G., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Veterinary Medicine, NAAS of Ukraine
    30, Donetska Str., Kyiv, Ukraine, 03151


The development of a DNA-vaccine against classical swine fever (CSF) is a perspective direction, because it gives an opportunity to develop a marker vaccine due to use of a part of protective antigen molecule, and to induce effectively both cellular and humoral immune response. In this study a recombinant plasmid, containing the fragment of E2 gene of CFS virus (CSFV) in eukaryotic expression vector, has been developed. It has been demonstrated that the fragment of E2 protein of CSFV is expressed in CHO-Kl cells from the developed recombinant plasmid pTR-BKneo , and we suggest that the protein possesses the post-translational modiВ­fications. The data obtained are in favor of the created model DNA-vaccine able to induce humoral immune response to fragment of E2 protein of CSFV.
Keywords: CSFV, DNA-vaccine, E2 glycoprotein of CSFV, immunization, humoral immune response


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