Stability of the genome of highly productive Rauwolfia serpentina Benth K-27 cell line at changing maintenance conditions

Spiridonova, K. V.; Adnof, D. M.; Andreev, I. O.; Kunakh, V. A.

doi:10.7124/bc.000759

Biopolym. Cell. 2007; 23(2):86-92.

http://dx.doi.org/10.7124/bc.000759

Molecular and Cell Biotechnologies

Stability of the genome of highly productive Rauwolfia serpentina Benth K-27 cell line at changing maintenance conditions

1Spiridonova K. V., 1Adnof D. M., 1Andreev I. O., 1Kunakh V. A.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Genetic analysis of the tissues of long-term passaged strain K-27 of Rauwolfia serpentina, maintained as surface or submerged cultures on the media of different composition for 4 passages, has been carried out via RAPD-PCR. Changes in the maintenance conditions were found to result in minor rearrangements of strain K-27 genome at the molecular level. However, the switch from the surface maintenance to the submerged one appears to affect the genome of cultured tissues more significantly as compared with the changes in the nutrient medium composition. Introduction of the additional subculture on the agarized medium of the intermediary composition preceding the switch to submerged culture seems to allow reducing the level of genome changes.

Keywords: plant tissue culture, Rauwolfia serpentina, plant genome, RAPD-analysis

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