Biopolym. Cell. 2007; 23(1):14-20.
Cell Biology
Synthesis of 2’-5’-oligoadenylates and study on their effect on proliferation and migration of bone marrow stem cells of mice in vitro and in vivo
1Tkachuk Z. Yu., 1Dubey I. Ya., 1Yakovenko T. G., 1Semernikova L. I., 1Shapoval S. O., 1Artemenko V. S., 1Dubey L. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

2’-5’-Triadenylate and its 3’-epoxy analogue were synthesized by phosphotriester method in solution. These compounds are shown to accelerate the migration of the bone marrow stem cells in donor mice and their inclusion into the spleen of recipient mice upon syngenic transplantation in vivo. 2’-5’-oligoadenylates and their derivatives depending on their structure and concentration influence apoptosis and proliferation of mice bone marrow cells in vitro.
Keywords: 2’-5’-oligoadenylates, migration, oligonucleotide ana<->logues, stem cells, proliferation, apoptosis

References

[1] Domkus VS, Domkene VA, Shemyakevichens VI, Nemeykayte AI, Sadauskas PB. Effects of some drugs on hematopoietic stem cells. Stem cells and tumor growth. Kiev. Naukova Dumka. 1985;238-9.
[2] Lukash LL, Vasilovskaya SV. Mammalian stem cells in vitro as a basis for the development of new biotechnologies. Biopolym Cell. 2001; 17(3):203-211.
[3] Gurevich AE, Egorov IK, Kyayvaryanen AI, Luriya EA, Mikhaylova AA, Nezmin RS, perov RV, Rokhlin OV, Sidorova EV, Fridenshtein AYa, Chertkov IL. Problems of developmental biology. Immunegenesis and cell differentiation. M: Nauka, 1978; 103 p.
[4] Frindel E. Factors stimulating and inhibiting bone marrow stem cells of mice. Stem cells and tumor growth. Kiev. Naukova Dumka. 1981; 45-53.
[5] Player MR, Torrence PF. The 2-5A system: modulation of viral and cellular processes through acceleration of RNA degradation. Pharmacol Ther. 1998;78(2):55-113.
[6] Katayama Y, Battista M, Kao WM, Hidalgo A, Peired AJ, Thomas SA, Frenette PS. Signals from the sympathetic nervous system regulate hematopoietic stem cell egress from bone marrow. Cell. 2006;124(2):407-21.
[7] Shyu WC, Lin SZ, Lee CC, Liu DD, Li H. Granulocyte colony-stimulating factor for acute ischemic stroke: a randomized controlled trial. CMAJ. 2006;174(7):927-33.
[8] Adah SA, Bayly SF, Cramer H, Silverman RH, Torrence PF. Chemistry and biochemistry of 2',5'-oligoadenylate-based antisense strategy. Curr Med Chem. 2001;8(10):1189-212.
[9] Zaporozhan VN, Bazhira YuI. Stem cells. Odessa, 2004. 227 c.
[10] Pat. US 5571799 (2'-5')Oligoadenylate analogues useful as inhibitors of host-vs. graft responce. Z. Tkachuk, E. Kvasyuk, G. Matsuka, I. Mikhailopulo. Publ. 1996.
[11] Sidorik LL, Dubei IYa, Bobyk VI, Kozlov AV, Fadorkova OM, Kovenya TV, Ryabenko DV, Sergienko OV, Trunina IV, Pogrebnoi PV, MAtsuka GKh. Therapeutic effects of the action of various doses of 2'-5'-oligoadenylate on experimental myosin-induced myocardial damage. Dopovidi Nats Akad Nauk Ukrainy. 2001; (9):161-5.
[12] Kyasyuk EI, Kulak TI, Khripach NB, Mikhailopulo IA, Uhlmann E, Charubala R, et al. Nucleotides XXIV; 1 Preparative synthesis of trimeric (2?-5?) oligoadenylic acid . Synthesis. 1987;1987(06):535–41.
[13] Oligonucleotide synthesis: a practical approach. Ed. M. J. Gait. Oxford: IRL press, 1984. 218 p.
[14] Robins MJ, Fouron Y, Mengel R. Nucleic acid related compounds. 11. Adenosine 2',3'-ribo-epoxide. Synthesis, intramolecular degradation, and transformation into 3'-substituted xylofuranosyl nucleosides and the lyxo-epoxide. J Org Chem. 1974;39(11):1564-70.
[15] Till JE, McCulloch EA. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961;14:213-22.
[16] Kuznetsov SA. Maintenance of hematopoiesis and lymphopoiesis in cultures of stromal substrate. Methods for cell culture. L.: Nauka, 1988; 267.
[17] Handbook of Clinical Laboratory Methods. Ed. EA Kost. M.: Meditsina, 1975; 82 p.
[18] Efimov VA, Buryakova AA, Reverdatto SV, Chakhmakhcheva OG, Ovchinnikov YuA. Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method. Nucleic Acids Res. 1983;11(23):8369-87.
[19] Tkachuk ZYu, Tkachuk VV, Tkachuk LV, Semernikova LI, Matsuka GKh. Influence of 2', 5' oligoadenylates and theirs analogues on the cyclic nucleotides level in vivo and in vitro. Biopolym Cell. 2001; 17(5):411-6.
[20] Fedorov NA. Cyclic nucleotides and leucogenesis. Stem cells and tumor growth. Kiev. Naukova Dumka 1985; 224 p.