The sources of heterogeneity of embryo cell populations of mouse line BalB/c cultured in vitro

Glazko, T. T.; Iatsyshyna, A. P.; Pidpala, O. V.; Vavilina, I. V.; Lukash, L. L.

doi:10.7124/bc.000742

Biopolym. Cell. 2006; 22(5):350-354.

http://dx.doi.org/10.7124/bc.000742

Cell Biology

The sources of heterogeneity of embryo cell populations of mouse line BalB/c cultured in vitro

1Glazko T. T., 1Iatsyshyna A. P., 1Pidpala O. V., 1Vavilina I. V., 1Lukash L. L.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Detailed chromosomal analysis of embryo mouse cell line G1 on the 15^th passage of in vitro cultivation has been performed. Possible mechanisms of heterogeneity of the investigated cell line are discussed. Spontaneous chromosome G-staining has been observed in some metaphase plates. Premature disjunction of sister cen tromeres has been found, in comparison with other regions of sister chromatids. The distribution of rearranged chromosomes (including Robertsonian translocations), the presence of autonomous chromosome clusters, associated in pericentromeric regions, as well as despiralization of pericentromeric heterochromatin blocks designate the similarity of metaphase chromosomes of a novel mouse Gl cell line to embryonic stem cells and embryonic germ cells of rodents

Keywords: stem cells, embryonic cells, germ stem cells, karyotypic heterogeneity.

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