Cytogenetic analysis of the spontaneously immortalized mouse cell line G1

Iatsyshyna, A. P.; Pidpala, O. V.; Kochubey, T. P.; Lukash, L. L.

doi:10.7124/bc.00073C

Biopolym. Cell. 2006; 22(4):299-306.

http://dx.doi.org/10.7124/bc.00073C

Cell Biology

Cytogenetic analysis of the spontaneously immortalized mouse cell line G1

1Iatsyshyna A. P., 1Pidpala O. V., 1Kochubey T. P., 1Lukash L. L.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Cytogenetic analysis of the spontaneously immortalized mouse cell line G1 has been carried out on passages 15, 24, 53, 68, 104 and 140 of in vitro cultivation. G1 cells were revealed to have quantitative chromosome changes, different chromosomal aberrations, and a particularly high percentage of Robertsonian translocations. The heterogeneity of cell population was observed to diminish during long-term cell cultivation. The karyotypic evolution of cells lasted not less than 140 passages on the stage of formation.

Keywords: karyotypic evolution, chromosome aberration, Robertsonian translocation

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