Biopolym. Cell. 2006; 22(3):236-242.
Low-temperature stabilization of glucose oxidase as a component of biological sensor
- Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The effect of freeze-thawing with various cooling rates on structural state of glucose oxidase using low temperatures has been studied for searching the ways of long-term preservation of biosensors. Such cryoprotectors as glycerol, 1,2-propane diol and DMSO were used for complete preservation of the enzyme properties in the process of low temperature preservation. The glycerol and 1,2-propane diol proved to be the most suitable for low temperature preservation of the glucose oxidase sensor that enabled to protect not only the protein part of biosensor but the biosensor protective surface as well.
Keywords: glucose oxidase, biosensor, low temperatures, cryoprotectants
Full text: (PDF, in English) (PDF, in Ukrainian)
 Sanders CA, Rodriguez M Jr, Greenbaum E. Stand-off tissue-based biosensors for the detection of chemical warfare agents using photosynthetic fluorescence induction. Biosens Bioelectron. 2001;16(7-8):439-46.
 Pancrazio JJ, Whelan JP, Borkholder DA, Ma W, Stenger DA. Development and application of cell-based biosensors. Ann Biomed Eng. 1999;27(6):697-711. ttp://
 Wolfbeis OS, Oehme I, Papkovskaya N, Klimant I. Sol-gel based glucose biosensors employing optical oxygen transducers, and a method for compensating for variable oxygen background. Biosens Bioelectron. 2000;15(1-2):69-76.
 Ricci F, Moscone D, Tuta CS, Palleschi G, Amine A, Poscia A, Valgimigli F, Messeri D. Novel planar glucose biosensors for continuous monitoring use. Biosens Bioelectron. 2005;20(10):1993-2000.
 Dzyadevych SV. Amperometric biosensors. Modern technologies and commercial variants. Biopolym Cell. 2002; 18(5):363-76.
 Barham D, Trinder P. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst. 1972;97(151):142-5.
 Shul'ga AA, Soldatkin AP, El'skaya AV, Dzyadevich SV, Patskovsky SV, Strikha VI. Thin-film conductometric biosensors for glucose and urea determination. Biosens Bioelectron. 1994;9(3):217-23.
 Gouda MD, Singh SA, Rao AG, Thakur MS, Karanth NG. Thermal inactivation of glucose oxidase. Mechanism and stabilization using additives. J Biol Chem. 2003;278(27):24324-33.
 Gidalevitz D, Huang Z, Rice SA. Protein folding at the air-water interface studied with x-ray reflectivity. Proc Natl Acad Sci U S A. 1999;96(6):2608-11.
 Pat USA N 949757. Stabilization of enzymes during freezing. S. P. Fitzgerald, J. Campbell, J. K. Lamont, M. A. King. Publ. 2001.
 Chaniotakis NA. Enzyme stabilization strategies based on electrolytes and polyelectrolytes for biosensor applications. Anal Bioanal Chem. 2004;378(1):89-95.
 Rozanova ED, Naumenko EI, Moiseev VA, Nardid OA. [Structural and functional parameters of cytochrome oxidase in solution and in liposomes: effect of glycerol] . Probl Kriobiol. 1992;(1):32-5.
 Timchenko N, Rozanova ED, Kucherenko YuV. [Spectral properties of fetal hemoglobin after freezing and thawing in solutions of glycerol and 1, 2-propanediol]. Probl Kriobiol. 2001;(4):73-4.
 M?ller F, Mayhew SG, Massey V. On the effect of temperature on the absorption spectra of free and protein-bound flavines. Biochemistry. 1973;12(23):4654-62.