Biopolym. Cell. 2006; 22(3):236-242.
http://dx.doi.org/10.7124/bc.000736
Bioorganic Chemistry
Low-temperature stabilization of glucose oxidase as a component of biological sensor
1Grischenko V. I., 1Nardid O. A., 1Rozanova K. D., 1Schetinsky M. I., 1Naumenko E. I., 2Dzyadevych S. V.
  1. Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
    23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The effect of freeze-thawing with various cooling rates on structural state of glucose oxidase using low temperatures has been studied for searching the ways of long-term preservation of biosensors. Such cryoprotectors as glycerol, 1,2-propane diol and DMSO were used for complete preservation of the enzyme properties in the process of low temperature preservation. The glycerol and 1,2-propane diol proved to be the most suitable for low temperature preservation of the glucose oxidase sensor that enabled to protect not only the protein part of biosensor but the biosensor protective surface as well.
Keywords: glucose oxidase, biosensor, low temperatures, cryoprotectants
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