Biopolym. Cell. 2006; 22(2):143-148.
http://dx.doi.org/10.7124/bc.000729
Molecular and Cell Biotechnologies
Copolymeric hydrogel membranes for immobilization and cultivation of human stem cells
1Kosenko O. O., 2Lukash L. L., 1Samchenko U. M., 2Ruban T. A., 1Ulberg Z. R., 2Lukash S. I.
  1. Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine
    42, Vernadsky blvd., Kyiv, Ukraine, 03142
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A number of hydrogel membranes for stem cells cultivation has been synthesized on the basis of acrylamide, acrylonitrile, and acrylic acid by the method of radical copolymerization. The comparison has shown that the optimal parameters, determined by the immobilization of human mesenchymal stem cells, are inherent to nonionic copolymer hydrogels on acrylamide and acrylonitrile bases. The use of hydrogel with considerable acrylic acid content is limited by pH decrease in cultivation medium, which is incompatible with life activity maintenance of the cells under investigation.
Keywords: hydrogel, stem cells, immobilization, cultivation medium
Full text: (PDF, in English) (PDF, in Ukrainian)

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