Biopolym. Cell. 2006; 22(2):102-108.
http://dx.doi.org/10.7124/bc.000722
Structure and Function of Biopolymers
The research on properties of clots, formed out of desAA- and desAABB-fibrin with different surface structure
1Savchuk O. M., 1Chernyshov V. I., 1Volkov G. L.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

It has been shown that on the surface of fibrin clots, which are formed at the interface between two phases excluding interpenetration of one phase into another one, the structure is being formed (a surface layer), which differs from the clot structure. At the absence of the interface, the formation of the layer on fibrin clot is not formed. There is an assumption that the creation of this structure is connected with the disturbance of the process of lateral association of fibrillar structures in fibrin clot, which takes place at the interface. The surface layer is formed by protofibrillar structures, which are spread flat on the interface and form compact unregulated structure which can be seen on electron microscope photographs. The revealed structure peculiarities of fibrin clots, caused by their formation conditions (presence or absence of the surface layer), determine the manner of the clots interaction with the components of fibrinolytic system, which can be the reason of differences in the degree of the fibrin clots resistance in blood circulation.
Keywords: fibrinogen, fibrin clot, electron microscopy
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References

[1] Mosesson MW. Thrombin interactions with fibrinogen and fibrin. Semin Thromb Hemost. 1993;19(4):361-7.
[2] Belitser VA, Varetskaya TV. Molecular mechanisms of fibrin polymerization and anticoagulant action of fibrinogen and fibrin degradation product. Thrombosis and Thrombosis. Eds E. I. Chazov, V. N. Smirnov. New York; London, 1986: 1-32.
[3] Belitser VA. [Domains-large, functionally important blocks of fibrinogen and fibrin molecules]. Biokhimia Cheloveka i Zhyvotnyh. 1982.; 6: 38-63.
[4] M?ller MF, Ris H, Ferry JD. Electron microscopy of fine fibrin clots and fine and coarse fibrin films. Observations of fibers in cross-section and in deformed states. J Mol Biol. 1984;174(2):369-84.
[5] Medved' L, Ugarova T, Veklich Y, Lukinova N, Weisel J. Electron microscope investigation of the early stages of fibrin assembly. Twisted protofibrils and fibers. J Mol Biol. 1990;216(3):503-9.
[6] Alving BM, Weinstein MJ, Finlayson JS, Menitove JE, Fratantoni JC. Fibrin sealant: summary of a conference on characteristics and clinical uses. Transfusion. 1995;35(9):783-90.
[7] Brennan M. Fibrin glue. Blood Rev. 1991;5(4):240-4.
[8] Goins KM, Khadem J, Majmudar PA, Ernest JT. Photodynamic biologic tissue glue to enhance corneal wound healing after radial keratotomy. J Cataract Refract Surg. 1997;23(9):1331–8.
[9] van Overbeeke JJ, Cruysberg JR, Menovsky T. Intracranial repair of a divided trochlear nerve. Case report. J Neurosurg. 1998;88(2):336-9.
[10] Varetskaya TV. Microheterogenity of fibrinogen. Cryofibrinogen. Ukr Biokhim Zh. 1960; 32(1):13-24.
[11] Mosesson MW. The preparation of human fibrinogen free of plasminogen. Biochim Biophys Acta. 1962;57:204-13.
[12] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[13] Robbins KC, Summaria L. Plasminogen and plasmin. Methods Enzymol. 1976;45:257-73.
[14] Savchuk A, Makogonenko E, Chernyshov V, Cederholm-Williams S. Structure and resistance to plasminolysis of fibrin clots formed in tube or inside agar gel. XVth Int Congr. on Fibrinolysis and Proteolysis. Hamamatsu, 2000; Abstr. 111.
[15] Pozdniakova TM, Rybachuk VN, Vovk Ev. [Analysis of the fragment D-fibrin monomer complex formation by salting-out fractionation]. Biokhimiia. 1982;47(6):971-6.
[16] Carr ME Jr, Gabriel DA, McDonagh J. Influence of Ca2+ on the structure of reptilase-derived and thrombin-derived fibrin gels. Biochem J. 1986;239(3):513-6.
[17] Hantgan R, Fowler W, Erickson H, Hermans J. Fibrin assembly: a comparison of electron microscopic and light scattering results. Thromb Haemost. 1980;44(3):119-24.