Biopolym. Cell. 2006; 22(1):82-84.
Short Communications
Regulation of S6K1 subcellular localization by Casein kinase 2
1Panasyuk G. G., 1Nemazanyy I. O., 1Zhyvoloup A. M., 1Filonenko V. V., 1Gout I. T.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Casein Kinase 2 (CK2) is physiological binding partner of ribosomal protein S6 kinase 1 identified using of two-hybrid yeast system. The specificity of interaction between β-subunit of CK2 and S6K1 was confirmed in vitro, also it was shown that Ser17 of S6K1 can be phosphorylated by CK2. The presented data suggest that Ser17 phosphorylation is the important event of S6K1 export from the nucleus. Immunoprecipitation studies of S6K1 and CK2 from cytoplasmic fraction of NIH3T3 cells indicate the formation of protein complex S6K1/CK2 in the nucleus, but not in the cytoplasm. Further fluorescent microscopy studies demonstrated that phosphorylation mimicking mutant of S6K1 (S17E) is not accumulated in the nucleus through the activated export of kinase from the nucleus.
Keywords: S6K1, S6K2, CK2β, nuclear export


[1] Thomas G. The S6 kinase signaling pathway in the control of development and growth. Biol Res. 2002;35(2):305-13.
[2] Panasyuk GG, Nemzanyy IO, Zhyvoloup AM, Filonenko VV, Gout IT. The beta subunit of casein kinase 2 as a novel binding partner of the ribosomal protein S6 kinase 1. Biopolym Cell. 2005; 21(5):407-12.
[3] Savinska LO, Lyzogubov VV, Usenko VS, Ovcharenko GV, Gorbenko ON, Rodnin MV, Vudmaska MI, Pogribniy PV, Kyyamova RG, Panasyuk GG, Nemazanyy IO, Malets MS, Palchevskyy SS, Gout IT, Filonenko VV. Immunohistochemical analysis of S6K1 and S6K2 expression in human breast tumors. Eksp Onkol. 2004;26(1):24-30.
[4] Zhang X, Shu L, Hosoi H, Murti KG, Houghton PJ. Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture. J Biol Chem. 2002;277(31):28127-34.
[5] Nemazanyy I, Panasyuk G, Zhyvoloup A, Panayotou G, Gout IT, Filonenko V. Specific interaction between S6K1 and CoA synthase: a potential link between the mTOR/S6K pathway, CoA biosynthesis and energy metabolism. FEBS Lett. 2004;578(3):357-62.
[6] Zhyvoloup A, Nemazanyy I, Panasyuk G, Valovka T, Fenton T, Rebholz H, Wang ML, Foxon R, Lyzogubov V, Usenko V, Kyyamova R, Gorbenko O, Matsuka G, Filonenko V, Gout IT. Subcellular localization and regulation of coenzyme A synthase. J Biol Chem. 2003;278(50):50316-21.
[7] Kutay U, GГјttinger S. Leucine-rich nuclear-export signals: born to be weak. Trends Cell Biol. 2005;15(3):121-4.