Biopolym. Cell. 2006; 22(1):49-55.
http://dx.doi.org/10.7124/bc.00071A
Molecular Biophysics
The collective vibration modes of hydrogen bonds as a gauge of water states in fruits and leaves
1Kornienko M. E., 1Kutovyi S. Y., 1Piddubetska O. V., 1, 2Smoljar N. P.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033
  2. IWR, Heidelberg Universitat
    Im Neuenheimer Feld 368, 69120 Heidelberg, Deutschland

Abstract

The new conception of the collective vibration modes (CVM) of the water medium is used to study water states in tissues (leaves and fruit). The CVM of the hydrogen bond (HB) nets were studied by the methods of the Raman and infrared (IR) spectroscopy in the region of the low-frequency LO and TO modes and high-frequency summary tones of these modes with fundamental vibrations. The frequency and intensity changes in the collective modes in comparison with distilled water are indicative of the HB nets ordered or disordered. It was found that in plant tissue the water can be both in disordered state as compared to distilled water and in more collectivized state. The existence of four different water states in leaves has been established.
Keywords: Raman and IR spectroscopy, HB-nets, water states, collective modes
Full text: (PDF, in English)

References

[1] Kornienko ME. LOOTO splitting and collective vibration excitations in liquids and amorphous medium. Physics of liquid matter. Kiev, 1989. Is sue 17: 93.
[2] Kornienko ME. Low frequency collective modes of the HBBnet of water. Ukr Fiz J. 2002; 47: 361-4.
[3] Kornienko ME. Observation of the LOOTO splitting in Raman spectra of liquids. Ukr Fiz J 2001; 46: 546-52.
[4] Kornienko ME. Low frequency collective vibrational modes of water. Proc. Int. Bunsen Discus. Meet. “Metastable Water”: Abstracts. Schloss Nordkirchen, 1999: 42.
[5] Kornienko ME. Lowwfrequency collective vibrational modes of hydrogen bonds net as the key to interpretation of the vibration sectra of water. Proc. Int. Conf. “Probl ems of Opt ics”: Abstracts. Kyiv, 1999: 17.
[6] Kornienko ME. Trans verseelon gitudinal split up of acoustic and optical modes of hydrogen bonds system of water. Proc. Int. Conf. of Spectra l Line Shapes (ICSLSSXV, PhysikalischhTechnische Bundesanstalt): Abst racts. Berlin, 2000: 107.
[7] Kornienko ME, Loutsenko AV. Researching collective lowwfrequency modes as a new quantitative method of studying liquid structure. Proc. Int. Conf. “Physics of Liquid Matter: Modern Problems”: Abstracts. Kyiv, 2001: 151.
[8] Kornienko ME, Kutovyi SYu, Piddubetska OV. The peculiarities of Raman spectra of bioactive water. Proc. Int. Conf. “Physics of Liquid Matter: Modern Problems”: Abstracts. Kyiv, 2001: 152.
[9] Kornienko ME, Kutovyi SYu, Piddubetska OV, Smoljar NP. The collective vibration modes of hydrogen bonds as biometer of the states of water invegetal fruits and leaves. Proc. Int. Conf. “Botanikertagungg2 002”: Abstracts. Freiburg, 2002: 395.
[10] Raman spectroscopy of Gases and Liquids. Ed. A. Veber. Amsterdam: Springer, 1979. 376 p.
[11] Uritskiy VM, Muzatevskaya NI. Fractal structures and biology processes. Biomed. Inform. SSPetersburg.: SPIIIRAN, 1995: 84-129.
[12] Kornienko ME, Smoljar NP, Jahne B, Schurr U. Different water states in plant leaves. Image spectroscopy of leaves water. Proc. Int. Conf. “Botanikertagungg2002”: Abstracts. Freiburg, 2002: 239.
[13] Kornienko ME, Smoljar NP, Jahne B, Schurr V. Image spectroscopy and spectral determination of the water contents in plant leaves. Proc. Int. Conf. “Botanikertagungg2002”: Abstracts. Freiburg, 2002: 239-40.