Biopolym. Cell. 2005; 21(4):346-350.
http://dx.doi.org/10.7124/bc.0006FB
Molecular and Cell Biotechnologies
Multibiosensor on the basis of yeast cells for detection of glucose and sucrose in aqueous solutions
1Kukla A. L., 2Kanyuk M. I., 2Starodub M. F., 1, 3Nagornaya S. S.
  1. V. Ye. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine
    41, Prospect Nauki, Kyiv, Ukraine, 03028
  2. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601
  3. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

A multichannel biosensor based on capacitive structures of electrolyte-insulator-semiconductor type with ion-sensitive layer of silicon nitride and exchangeable alginate membranes with immobilized yeast cells Candida krusei UKM Y-823, Saccharomyces cerevisiae UKM Y-517 and Cryptococcus laurentii UKM Y-1135 for simultaneous determination of glucose and sucrose concentration has been developed. The multibiosensor operation is based on the selectivity of used yeast cells towards carbohydrates and proportional generation of acid when the carbohydrates are used by yeast cells as a substrate. The measurable glucose and sucrose concentration ranges are 2–30 mM and 2–50 mM, correspondingly. The accuracy of glucose and sucrose detection in these ranges is about 5–10 %.
Keywords: multibiosensor, yeast, glucose, sucrose
Full text: (PDF, in Ukrainian)

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