Biopolym. Cell. 2005; 21(3):275-282.
http://dx.doi.org/10.7124/bc.0006F2
Molecular and Cell Biotechnologies
Potentiometric biosensor for detection of potato glycoalcaloids: control of its analytical characteristics, comparison with thin-layer chromatography
1Nazarenko A. A., 1Soldatkin A. P., 1Sosovskaya O. F., 1Benilova I. V., 1Korpan Ya. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Butyrylcholinesterase (BuChE) of different origin along with variations of the time of the enzyme immobilization on the potentiometric transducer surface are offered to control analytical characteristics of the biosensor, based on pH-sensitive field effect transistor with immobilized BuChE, for detection of the potato glycoalcaloids. Utilization of ethylendiaminetetracetate (a complexon of heavy metal ions) and phosphotriesterase (an enzyme capable of exclusive disintegration of phosphororganic pesticides) is shown to enable the selective determination of glycoalcaloids at the background of heavy metal ions and phosphororganic pesticides. 14 potato varieties cultivated in Ukraine have been tested by the biosensor and thin-layer chromatography the correlation between both methods being 0.74.
Keywords: biosensor, glycoalcaloids, pH-sensitive field effect transistors, butyrylcholinesterase, thin-layer chromatography
Full text: (PDF, in Ukrainian)

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