Biopolym. Cell. 2005; 21(3):270-274.
Molecular and Cell Biotechnologies
Development of model DNA-vaccine
1Pokholenko I. O., 1Titok T. G., 1Sukhorada O. M., 1Ruban T. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The development of DNA-vaccines is one of the most perspective directions in modern vaccionology. However, despite rapid progress in their application, a broad range of questions concerning the basic mechanisms of induction of immune response to DNA-vaccination remains unanswered. A model DNA-vaccine, containing the gene of Escherichia coli β-galactosidase as the gene of model antigen, has been developed. The data obtained suggest that the created recombinant plasmid pBG-TR/02 is able to induce the humoral immune response to E. coli β-galactosidase in mice depending on the place of administration and, therefore, it can be used as the model DNA-vaccine for future research.
Keywords: DNA-vaccine, model antigen, immunization, humoral immune response


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