Biopolym. Cell. 2005; 21(3):258-263.
http://dx.doi.org/10.7124/bc.0006EF
Viruses and Cell
Extraction of intercellular glycobiopolymers from leaves of hypersensitive tobacco plants infected by tobacco mosaic virus
1Kovalenko O. G., 1Kyrychenko A. M., 1Telegeeva T. A.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Accumulation of intercellular glycobiopolymers has been discovered at early infection stages (one day after inoculation) in apoplast of hypersensitive tobacco plants infected by tobacco mosaic virus. The two components, absent in control, were extracted by gel-filtration (on bio-gel P-10), ion-exchange (on DEAE-Sephadex) and affinity (on concanavalin A) chromatography methods. The participation of discovered components in signal transfer from infected cells to healthy ones is postulated. Due to the signal, the mechanisms are initiated to localize the virus infection and/or to develop the induced resistance of plant tissue to reinfection.
Keywords: tоbacco mosaic virus, hypersensitive reaction, glycobiopolymers, concanavalin A, distribution and affinity chromatography
Full text: (PDF, in Ukrainian)

References

[1] Kovalenko OH, Kyrychenko AM, Teleheieva TA. [Effect of TMV-infection on protein and carbohydrate content in hypersensitive tobacco plants and their antiviral and hemagglutinating activity]. Ukr Biokhim Zh. 2003;75(2):103-8. Ukrainian.
[2] Kovalenko OG, Kyrychenko AM, Telegeeva TA. The affinity chromatography of glicobiopolymers from sensitive and hypersensitive tobacco plants infected by tobacco mosaic virus. Biopolym Cell. 2005; 21(2):151-6.
[3] Pierpoint WS. The major proteins in extracts of tobacco leaves that are responding hypersensitively to virus-infection. Phytochemistry. 1983;22(12):2691–7.
[4] Wieringa-Brants DH, Dekker WC. Induced resistance in hypersensitive tobacco against tobacco mosaic virus by injection of intercellular fluid from tobacco plants with systemic acquired resistance. J Phytopathol. 1987;118(2):165–70.
[5] Kovalenko AG. Protein-carbohydrate interactions in the realization of plant resistance to viruses. Mikrobiol Zh. 1993; 55(6):74-91.
[6] Kovalenko OH, Kyrychenko AM. [TMV-infection localization and development of induced virus resistance in Nicotiana sanderae Hort., Datura stramonium L. and Datura metel]. Mikrobiol Z. 2004;66(4):43-7.
[7] Dangl JL, Jones JD. Plant pathogens and integrated defence responses to infection. Nature. 2001;411(6839):826-33.
[8] Jones DA, Jones JDG. The role of leucine-rich repeat proteins in plant defences. advances in botanical research. 1997;89–167.
[9] Kajava AV. Structural diversity of leucine-rich repeat proteins. J Mol Biol. 1998;277(3):519-27.
[10] Goldbach R, Bucher E, Prins M. Resistance mechanisms to plant viruses: an overview. Virus Res. 2003;92(2):207-12.