Investigation of some structural-functional peculiarities of the immunogenic fragment Ki-67 antigen. Generation of polyclonal and monoclonal antibodies to Ki-67

Tykhonkova, I. O.; Lisogubov, V. V.; Khozhaenko, Yu. V.; Dovgopola, O. O.; Korovin, S. I.; Ovcharenko, G. V.; Usenko, V. S.; Rodnin, M. V.; Filonenko, V. V.

doi:10.7124/bc.0006EA

Biopolym. Cell. 2005; 21(2):180-183.

http://dx.doi.org/10.7124/bc.0006EA

Short Communications

Investigation of some structural-functional peculiarities of the immunogenic fragment Ki-67 antigen. Generation of polyclonal and monoclonal antibodies to Ki-67

1Tykhonkova I. O., 2Lisogubov V. V., 2Khozhaenko Yu. V., 3Dovgopola O. O., 3Korovin S. I., 1Ovcharenko G. V., 2Usenko V. S., 1Rodnin M. V., 1Filonenko V. V.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Pathomorphological Laboratory "BIONTEK"
52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000
Institute of Oncology of Academy of Medical Sciences of of Ukraine
Street. University, 33/43, Kiev, Ukraine, 03022

Abstract

The study of tumor-associated antigens function is a prerequisite for understanding the molecular mechanisms of oncogenesis. Earlier we have determined the nuclear protein Ki-67 as an autoantigen in human melanoma. cDNA of immunogenic fragment of Ki-67 has been cloned into pET23d vector and expressed in bacterial cells. The recombinant fragment of Ki-67 has been purified from the bacterial cell lysate has been used as an antigen for the production of polyclonal and monoclonal antibodies with following detection of Ki-67 in Breast adenocarcinomas. We have shown by the immunodot-blot analysis that the 18 kDa recombinant fragment of Ki-67 possesses a DNA-binding activity.

Keywords: Ki-67 antigen, antibodies, immunohistochemistry, DNA-binding activity

Full text: (PDF, in Ukrainian)

References

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