Biopolym. Cell. 2005; 21(2):151-156.
Viruses and Cell
The affinity chromatography of glicobiopolymers from sensitive and hypersensitive tobacco plants infected by tobacco mosaic virus
- D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680
The extracellular carbohydrate-containing biopolymers which are absent in healthy plants have been discovered in hypersensitive plants by the affinity chromatography method one day after inoculation Such components have not been revealed in tissues of TMV-infected sensitive mutant of the same variety. Since these biopolymers may be specifically bound to concanavalin A, but not to germ wheat agglutinin, they probably contain the mannosa and/or glucosa as carbohydrate components. A possible functional role of these biopolymers in initiation of virus-induced hypersensitive reaction in plants is discussed.
Keywords: tobacco mosaic virus, hypersensitive reaction, glicobiopolymers, lectins, affinity chromatography
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 Sela I. Plant-virus interactions related to resistance and localization of viral infections. Adv Virus Res. 1981;26:201-37.
 Loebenstein G, Gera A. The local lesion response to viruses: possibilities for engineering resistant plants. Biotechnology in Plant Disease Control. Lissabon: Wiley, 1993: 105-13.
 Wieringa-Brants DH, Dekker WC. Induced Resistance in Hypersensitive Tobacco Against Tobacco Mosaic Virus by Injection of Intercellular Fluid from Tobacco Plants with Systemic Acquired Resistance. J Phytopathol. 1987;118(2):165вЂ“70.
 Gianinazzi S. Antiviral agents and inducers of virus resistance: analogies with interferon. Active Defence Mechanisms in Plants. Ed. R. K. S. Wood. New York; London: Plenum Press, 1982: 275-98.
 Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B. The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell. 1994;78(6):1101-15.
 Kovalenko AG. Protein-carbohydrate interactions in the realization of plant resistance to viruses. Mikrobiol Zh. 1993; 55(6):74-91.
 Kovalenko OG, Telegeeva TA, Shtakun AV, Pogorila ZO. Influence of some mono- and polysaccharides on localization of virus infection and on induced virus resistance in plants. Biopolym Cell. 2000; 16(1):53-9.
 Kovalenko OG, Telegeeva TA, Kyrychenko AM. Discovery of lectin-like proteins in the tobacco and datura plants, infected by tobacco mosaic virus. Biopolym Cell. 2003; 19(2):164-8.
 Kovalenko OH, Kyrychenko AM, Teleheieva TA. [Effect of TMV-infection on protein and carbohydrate content in hypersensitive tobacco plants and their antiviral and hemagglutinating activity]. Ukr Biokhim Zh. 2003;75(2):103-8.
 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
 Zakharova IYa, Kosenko LB. Methods for studying microbial polysaccharides. Kiev: Naukova Dumka. 1982. 163 p.
 Kovalenko AG, Barkalova AA. Production of biologically active mannan containing preparations from yeast. Mikrobiol Zh. 1995; 57(3):41-7.
 Whistler RL, Wolfrom ML. Methods in Carbohydrate Chemistry Academic Press, New York-London 1964.
 Kovalenko AG, Kovalenko EA, Grabina TD. Lectine-binding and antiviral activity of the yeast mannanes in supersensitive plants. Mikrobiol Zh. 1991; 53(2):83-9.
 Casalongue C, Lezica R. Potato lectin: A cell-wall glucoprotein. Plant Cell Physiol. 1986; 26(8): 1533-9.
 Khomutovskiy OA, Lutsik MD, Perederey OF. Electronic histochemistry cell membrane receptors. Kiev: Naukova Dumka, 1986. 167 p.
 Turkova J. Affinity Chromatography. Elsevier Science Technol, 1978.
 Allan AC, Lapidot M, Culver JN, Fluhr R. An early tobacco mosaic virus-induced oxidative burst in tobacco indicates extracellular perception of the virus coat protein. Plant Physiol. 2001;126(1):97-108.