Biopolym. Cell. 2005; 21(2):140-144.
Cell Biology
Autotransformation of mammalian cells
1Kordium V. A., 1Shpilevaya S. P., 1Ruban T. A., 1Sukhorada O. M., 1Andriyenko V. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The experiments to prove genetic material transfer between mammalian cells have been planned and performed. They show the existence of such transfer with subsequent expression of the marker genes in the recipient cells. The transfer takes place in physiological conditions without any outside influence. This gives the possibility to consider such transfer as a normal process, and the exchange of genetic material between mammalian cells as one of the components of the intercellular interactions.
Keywords: spontaneous transformation, mammalian cells, transfer of DNA, marked lines


[1] Lipskaia LA, Zhitkovich AV, Vasiukhin VI, Tsvetkov AG, Sal'nikov KV. [The participation of endonuclease in the formation of extrachromosomal DNA and the possible mechanisms of the occurrence of gene amplification]. Tsitologiia. 1993;35(1):70-8.
[2] Anker P, Stroun M, Maurice PA. Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system. Cancer Res. 1975;35(9):2375-82.
[3] Stroun M, Anker P, Maurice P, Gahan PB. Circulating nucleic acids in higher organisms. Int Rev Cytol. 1977;51:1-48.
[4] Stroun M, Anker P, Lyautey J, Lederrey C, Maurice PA. Isolation and characterization of DNA from the plasma of cancer patients. Eur J Cancer Clin Oncol. 1987;23(6):707-12.
[5] Fedorov NA, Ianeva IS. DNA excretion by human lymphocytes. Usp Sovrem Biol. 1982;93(2):171-82.
[6] Kazakov VI, Bozhkov VM, Linde VA, Repina MA, Mikha?lov VM. Extracellular DNA in the blood of pregnant women. Tsitologiia. 1995;37(3):232-6.
[7] Vladimirov VG, Sherlina SS. [Effect of extreme factors, differing in nature, on distribution of repetitive sequences in extracellular DNA]. Radiats Biol Radioecol. 2002;42(6):754-8.
[8] Bergsmedh A, Szeles A, Henriksson M, Bratt A, Folkman MJ, Spetz AL, Holmgren L. Horizontal transfer of oncogenes by uptake of apoptotic bodies. Proc Natl Acad Sci U S A. 2001;98(11):6407-11.
[9] Bennett RM, Gabor GT, Merritt MM. DNA binding to human leukocytes. Evidence for a receptor-mediated association, internalization, and degradation of DNA. J Clin Invest. 1985;76(6):2182-90.
[10] Emlen W, Rifai A, Magilavy D, Mannik M. Hepatic binding of DNA is mediated by a receptor on nonparenchymal cells. Am J Pathol. 1988;133(1):54-60.
[11] Loke SL, Stein CA, Zhang XH, Mori K, Nakanishi M, Subasinghe C, Cohen JS, Neckers LM. Characterization of oligonucleotide transport into living cells. Proc Natl Acad Sci U S A. 1989;86(10):3474-8.
[12] Takagi T, Hashiguchi M, Mahato RI, Tokuda H, Takakura Y, Hashida M. Involvement of specific mechanism in plasmid DNA uptake by mouse peritoneal macrophages. Biochem Biophys Res Commun. 1998;245(3):729-33. PubMed PMID: 9588183.
[13] Al-Mousa S, Nicholls PJ. Gumbleton M. Evidence for the role of caveolae in gene delivery. J Pharm Pharmacol. 1999; 51: 178-83.
[14] Siess DC, Vedder CT, Merkens LS, Tanaka T, Freed AC, McCoy SL, Heinrich MC, Deffebach ME, Bennett RM, Hefeneider SH. A human gene coding for a membrane-associated nucleic acid-binding protein. J Biol Chem. 2000;275(43):33655-62.
[15] Li X, Sambhara S, Li CX, Ewasyshyn M, Parrington M, Caterini J, James O, Cates G, Du RP, Klein M. Protection against respiratory syncytial virus infection by DNA immunization. J Exp Med. 1998;188(4):681-8.
[16] Triyatni M, Jilbert AR, Qiao M, Miller DS, Burrell CJ. Protective efficacy of DNA vaccines against duck hepatitis B virus infection. J Virol. 1998;72(1):84-94.
[17] Tiollais P, Michel ML. Genetic vaccination. Perspectives for the prevention and treatment of hepatitis B. C R Acad Sci III. 1999;322(11):979-81.
[18] Weiner DB, Kennedy RC. Genetic vaccines: Vaccines crafted from genetic material might one day prevent AIDS, malaria and other devastating infections that defy current immunization technologies. They may even help treat cancers. Sci Amer. 1999; 281(1):34-41.
[19] Gurunathan S, Klinman DM, Seder RA. DNA vaccines: immunology, application, and optimization*. Annu Rev Immunol. 2000;18:927-74.
[20] Ishii KJ, Suzuki K, Coban C, Takeshita F, Itoh Y, Matoba H, Kohn LD, Klinman DM. Genomic DNA released by dying cells induces the maturation of APCs. J Immunol. 2001;167(5):2602-7.
[21] Kordyum VA, Toporova EK, Okunev OV, Pokholenko YA, Suchorada EM., Ruban TA, Andrienko VI, Irodov DM. Novel cell line with multiple markers, derivative of CHO-K1. Biopolym Cell. 2003; 19(3):252-6.
[22] Kordyum VA. Our "Shagreen leather" is our problem. And we have to solve it. 3. A missing link. Biopolym Cell. 2003; 19(6):473-91.