Biopolym. Cell. 2005; 21(1):35-41.
Cell Biology
Intertribal somatic hybrids of di- and tetragenome origin: production and usage
1Ovcharenko O. O., 1Komarnytskyy I. K., 1Cherep M. M., 1Rudas V. A., 1Kuchuk M. V.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680


Intertribal hybrids in the combinations of Orychophragmus violaceus (L.) O. E. Schulz. + Arabidopsis thaliana L. and O. violaceus (L.) O. E. Schulz. + hybrid (Brassica juncea (L.) Czern. & Coss + A. thaliana L.) were obtained as a result of somatic hybridization. Hybrid nature of the plants obtained was confirmed by the PCRRFLP and analysis of isozymes. A. thaliana L. plants were transformed with a vector carrying heterologous transposable ele­ment SpmldSpm and genes nptll, bar, gus, which allowed to examine the behavior of transgenic traits in digenomic and tetragenomic hybrids. The hybrid plants were similar to one of the parental species (O. violaceus + A. thaliana) or had intermediate morphology (O. violaceus + hybrid (B. juncea + A. thaliana)). All the plants obtained were resistant to kanamycin and phosphinothricin due to the transgenes transferred from A. thaliana The activity of β-glucuronidase was detected in hybrid O. violaceus + + A. thaliana while it was absent in the plants O. violaceus + hybrid (B. juncea + A. thaliana). PCR analysis revealed maintenance of all transgenes in the hybrids.
Keywords: intertribal somatic hybridization, Orychophragmus violaceus, Brassica juncea, Arabidopsis thaliana, heterologous sys­tem of transposable element Spm/dSpm


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