Biopolym. Cell. 2004; 20(6):498-504.
Cell Biology
Subcellular localization and activity of phosphorylase in parenchyma cells of Solanum tuberosum L. minitubers at clinorotation
1Nedukha O. M., 1Schnyukova E. I.
  1. M. G. Kholodny Institute of Botany, NAS of Ukraine
    2, Tereschenkivska Str., Kyiv, Ukraine, 01601


The subcellular localization and the phosphorylase (EC activity in parenchyma cells of Solanum tuberosum L. (sv Adreta) minitubers have been investigated. The minitubers were formed in the stationary control and under influence of horizontal clinorotation, which imitated microgravity. The next methods used in the experiments: i) the method of sterial culture of miniplants, ii) electronic cytochemical method, and iii) biochemical methods for the determination of carbohydrates content, phosphorylase activity, and isoenzyme composition of phosphorylase. It is established that clinorotation does not influence the subcellular localization of phosphorylase and the electrophoretic mobility of phosphorylase isoforms; however, the clinorotation increases the phosphorylase activity and starch content in potato storage organs.


[1] Kordyum EL. Biology of plant cells in microgravity and under clinostating. Int Rev Cytol. 1997; 171:1-78.
[2] Sytnik KM, Kordyum YeL, Nedukha OM, Fomicheva VM. Plant cell when the geophysical factors. Kiev, Naukova Dumka, 1984; 134 p.
[3] Kuznetsov O, Brown C, Levine H, Sanwo M, Hasensteinn K. Space-grown plants show modified starch structure: Abstr. of 33rd COSPAR 2000 (Warsaw, 16-28 July). Warsaw, 2000; 631 p.
[4] Nedukha EM. Effects of microgravity on the structure function of plant cell walls. Int Rev Cytol. 1997; 170:39-77.
[5] Jiao S, Hilaire E, Paulsen AQ, Guikema JA. Ultrastructural observation of altered chloroplast morphology in space-grown Brassica rapa cotyledons. J Gravit Physiol. 1999; 6 (1):93-4.
[6] Popova AF, Kordyum EL, Shnyukova EI, Sytnik KM. Plastid ultrastructure, fractional composition and activity of amylases in Chlorella cells in microgravity. J Gravit Physiol. 1995; 2 (1):159-60.
[7] Smith AM, Denyer K, Martin C. The synthesis of the starch granule. Annu Rev Plant Biol. 1997; 48:67-87.
[8] Steup M, Peavey DG, Gibbs M. The regulation of starch metabolism by inorganic phosphate. Biochem Biophys Res Commun. 1976; 72 (4):1554-61.
[9] Heldt HW, Chon CJ, Maronde D. Role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiol. 1977;59(6):1146-55.
[10] Watson KA, McCleverty C, Geremia S, Cottaz S, Driguez H, Johnson LN. Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: Answers to a long outstanding question. EMBO J. 1999; 18 (17):4619-32.
[11] Croxdale J, Cook M, Tibbitts TW, Brown CS, Wheeler RM. Structure of potato tubers formed during spaceflight. J Exp Bot. 1997; 48 (317):2037-43.
[12] Murashige T, Skoog FA. A revised medium of rapid growth and bioassays with tobacco tissue culture. Physiol Plantar. 1962; 15 (3):473-97.
[13] Nedukha EM. Location of phosphorylase activity in protonema cells of Funaria hygrometrica Hedw. Tsitiligiia. 1977; 19(9): 1062-1064.
[14] Yermakov AI, Arasimovich VV, Yarosh YaYa, Peruanskiy YuV, Lukovnikov GA, Ikonnikova MI. Methods biochemical study of plants. Ed. AI Ermakov. Leningrad, Agropromizd. 1987; 430 p.
[15] Rodionova V, Kholoptseva M. Determination of phospholipids leaves dimensional thin-layer chromatography silica gel. Plant Physiol. 1974; 6(2):201-204.
[16] Gerbrandy SjJ, Verleur JD. Phosphorylase isoenzymes: Localization and occurrence in different plant organs in relation to starch metabolism. Phytochemistry. 1971; 10 (2):261-6.
[17] Bolotova VTs, Sakanen YeI, Lesiovskaya YeYe, Pastushenkov LV. Spectrophotometric method for determination of polysaccharides in the leaves of Tilia cordata. Plant Resources. 2001; 37(3):109-112.
[18] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193 (1):265-75.
[19] Perbal G, Driss-Ecole D, Rutin J, Salle G. Graviperception of lentil seedling roots grown in space (Spacelab D1 Mission). Physiol Plantar. 1987; 70: 119-26.
[20] Geigenberger P, Hajirezaei M, Geiger M, Deiting U, Sonnewald U, Stitt M. Overexpression of pyrophosphatase leads to increased sucrose degradation and starch synthesis, increased activities of enzymes for sucrose-starch interconversions, and increased levels of nucleotides in growing potato tubers. Planta. 1998; 205 (3):428-37.
[21] Xu X, Van Lammeren AAM, Vermeer E, Vreugdenhil D. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol. 1998; 117 (2):575-84.
[22] Vreugdenhil D, Struik P. An integrated view of the hormonal regulation of tuber formation in piotato (Solanum tuberosum). Physiol. Plantar. 1989; 75: 525-31.
[23] Albrecht T, Koch A, Lode A, Greve B, Schneider-Mergener J, Steup M. Plastidic (Pho1-type) phosphorylase isoforms in potato (Solanum tuberosum L.) plants: Expression analysis and immunochemical characterization. Planta. 2001; 213 (4):602-13.
[24] Kursanov AL. Transport of assimilates in the plant. Moscow, Nauka. 1976; 646 p.