Biopolym. Cell. 2004; 20(6):493-497.
Structure and Function of Biopolymers
Purification and properties of the lectins from aerial part of three species of plants genus hawkweed (Hieracium)
1Antonyuk V. A.
  1. Institute of Cell Biology, NAS of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005


From aerial part of three species of the plants of genus hawkweed (Hieracium aurantiacum, H. pilosella, H. sylvularum), family Asteraceae, the lectins have been purified with the yield of 61, 126 and 92 mg per kg of crude drugs, accordingly. The molecular mass of the three lectins as determined by gel filtration on Toyopearl HW-55 is 36 kDa. According to the SDS-PAGE data, the lectins contain one component of molecular mass approximately 18 kDa. The PAGE disc electrophoresis in an alkaline (pH 8.9) and acidic systems (pH 4.3) detected one protein band, that presumes the lectins investigated to consist of one isoform. The sugar component (2.1–2.2 %) was revealed in the lectins. The lectins of three species studied agglutinate rabbit erythrocytes best of all and agglutinate weaker hen, sheep and human erythrocytes. The best inhibitor of lectin activity for three species of genus hawkweed is N,N'‚N'''‚N'''-tetraacethylchitotetraose. This sugar is 8 time stronger inhibitor than N,N'-diacethylchitobiose and 625 time stronger than N-acethyl-D-glucosamine. Peroxidase-lectin conjugates and FITC-labelled lectins for histochemicals applications have been prepared.


[1] Peumans WJ, Van Damme EJ. Lectins as plant defense proteins. Plant Physiol. 1995;109(2):347-52.
[2] Lakhtin VM, Yamskov IA. Lectins in the investigation of receptors. Russ Chem Rev. 1991; 60 (8):903-23.
[3] Roth J, Zuber C, Komminoth P, Sata T, Li WP, Heitz PU. Applications of immunogold and lectin-gold labeling in tumor research and diagnosis. Histochem Cell Biol. 1996;106(1):131-48.
[4] Gornik I, Maravic G, Dumic J, Flogel M, Lauc G. Fucosylation of IgG heavy chains is increased in rheumatoid arthritis. Clin Biochem. 1999;32(8):605-8.
[5] Lutsik MD, Antonyuk VA. Preparation of anti-A reagent of pea lectin shaggy to detect antigen A in erythrocytes and human saliva. ekspertiza.1995; 1:17-9.
[6] Lutsik AD, Detyuk YeS, Lutsik MD. Lectin histochemistry. Lviv, Izd. L'viv Univ, 1989. 144 p.
[7] Determinant of higher plants Ukraine. Ed. N Prokudin. Kyiv: Naukova Dumka, 1987; 389 p.
[8] Bourne Y, Zamboni V, Barre A, Peumans WJ, Van Damme EJ, Rouge P. Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectins. Structure. 1999;7(12):1473-82.
[9] Antonyuk VO, Rybak OV. Study of lectin-carbohydrate spetsyfychnosti ground of Echinacea purpurea and rudbeckia rozdilnolystoyi. Farmakom. 2002; 3:153-8.
[10] Antonyuk VO, Dubyts'kyy OL. Study of carbohydrate specificity of lectins from plants of the genus Artemisia. Ukr Biokhim Zh. 2002;74(4B Suppl 2):114.
[11] A. p. USSR number 1554961. A method for producing affinity sorbent for purification of lectins. VA Antonyuk. Byul. izobreteniy. 1990. N 13.
[12] Read SM, Northcote DH. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981;116(1):53-64.
[13] Lutsik MD, Panasyuk EH, Antonyuk VA, Lutsik AD, Ladnaya LYa. Research methods carbohydrate specificity of lectins (Method. recommendations). Lviv, 1983. 22 p.
[14] Maurer HR. Disc electrophoresis and related techniques of polyacrylamide gel electrophoresis. de Gruyter, 1971; 222 p.
[15] Kato Y., Komiya K., Iwaed T., Sasaki H., Hashimoto T. Packing of toyopearl column for gel filtration. I. Influence of packing velocity on column performance. J Chromatogr A. 1981; 205 (1):185-188.
[16] Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28 (3): 350-356.
[17] Nakane PK, Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974;22(12):1084-91.
[18] Antoniuk VA, Iashchenko AM. Conjugation of lectins with horseradish peroxidase: an improved method. Klin Lab Diagn. 1996;(3):51-2.