Biopolym. Cell. 2004; 20(5):416-420.
Genome and Its Regulation
Search for possible changes in murine gene Cyp2e1 structural region under exposure to ionizing radiation
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The structural region of murine Cyp2e1 gene was cloned in Escherichia coli system. We identified a nucleotide sequence of gene exon regions of 1482 base pairs. Substitutions, inserts and deletions in the gene structural region revealed by sequencing appeared to be invalid. Thus, low doses of Chernobyl specter radiation do not cause changes in the nucleotide sequence of structural region of murine cytochrome P450 2E1 gene.
 Parke DV, Ioannides C, Lewis DF. The 1990 Pharmaceutical Manufacturers Association of Canada keynote lecture. The role of the cytochromes P450 in the detoxication and activation of drugs and other chemicals. Can J Physiol Pharmacol. 1991;69(5):537-49.
 Lieber CS. Cytochrome P-4502E1: its physiological and pathological role. Physiol Rev. 1997;77(2):517-44.
 Gergel D, Misik V, Riesz P, Cederbaum AI. Inhibition of rat and human cytochrome P4502E1 catalytic activity and reactive oxygen radical formation by nitric oxide. Arch Biochem Biophys. 1997;337(2):239-50.
 Morel Y, de Waziers I, Barouki R. A repressive cross-regulation between catalytic and promoter activities of the CYP1A1 and CYP2E1 genes: role of H(2)O(2). Mol Pharmacol. 2000;57(6):1158-64.
 Maksymchuk OV, Danko IM, Rosohac'ka IV, Chaschyn MO. Gene Cyp2e1 expression in mouse liver cells exposed to low-dose radiation. Biopolym Cell. 2002; 18(6):518-21.
 Maksymchuk OV, Danko IM, Chashchin MO. Variation in the levels of expression and content of cytochrome P450 2E1 (Cyp2e1) under the action of ionizing radiation. Dopovidi Nats Akad Nauk Ukrainy. 2003; (11):162-6.
 Chung HC, Kim SH, Lee MG, Cho CK, Kim TH, Lee DH, Kim SG. Mitochondrial dysfunction by gamma-irradiation accompanies the induction of cytochrome P450 2E1 (CYP2E1) in rat liver. Toxicology. 2001;161(1-2):79-91.
 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-9.
 Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual New York: Cold Spring Harbor Lab. publ., 1982 545 p.
 Nishimura A, Morita M, Nishimura Y, Sugino Y. A rapid and highly efficient method for preparation of competent Escherichia coli cells. Nucleic Acids Res. 1990;18(20):6169.
 Novak RF, Woodcroft KJ. The alcohol-inducible form of cytochrome P450 (CYP 2E1): role in toxicology and regulation of expression. Arch Pharm Res. 2000;23(4):267-82.
 Emoto C, Yamazaki H, Yamasaki S, Shimada N, Nakajima M, Yokoi T. Characterization of cytochrome P450 enzymes involved in drug oxidations in mouse intestinal microsomes. Xenobiotica. 2000;30(10):943-53.
 Ingelman-Sundberg M, Johansson I, Yin H, Terelius Y, Eliasson E, Clot P, Albano E. Ethanol-inducible cytochrome P4502E1: genetic polymorphism, regulation, and possible role in the etiology of alcohol-induced liver disease. Alcohol. 1993;10(6):447-52.
 Freeman JE, Stirling D, Russell AL, Wolf CR. cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1. Biochem J. 1992;281 ( Pt 3):689-95.
 Song BJ, Gelboin HV, Park SS, Yang CS, Gonzalez FJ. Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme. J Biol Chem. 1986;261(35):16689-97.
 Umeno M, McBride OW, Yang CS, Gelboin HV, Gonzalez FJ. Human ethanol-inducible P450IIE1: complete gene sequence, promoter characterization, chromosome mapping, and cDNA-directed expression. Biochemistry. 1988;27(25):9006-13.
 Lundberg KS, Shoemaker DD, Adams MW, Short JM, Sorge JA, Mathur EJ. High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 1991;108(1):1-6.