Biopolym. Cell. 2004; 20(5):410-415.
Structure and Function of Biopolymers
DNA denaturation during senescence of soybean pod valve
- Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17, Vasylkivska, Kyiv, Ukraine, 03022
Abstract
During senescence of pod valve of soybean (Glycine max L., Merr.) no olygonucleocomic DNA fragmentation is observed and total DNA is present as high polymeric fragments with an equal molecular mass, which, nevertheless, are subjected to partial denaturation in the course of dehydration and chlorophyll disruption. These data are in favour of a conception that senescence of soybean pod valve may be considered as some form of programmed cell death (PCD). Also, they evidence the differential processes of DNA degradation during the plant ontogenesis PCD.
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References
[1]
Greenberg JT. Programmed cell death: A way of life for plants. Proc Nat Acad Sci USA. 1996; 93 (22):12094-7.
[3]
Orzaez D, Granell A. DNA fragmentation is regulated by ethylene during carpel senescence in Pisum sativum. Plant J. 1997; 11 (1):137-44.
[4]
Yoshida A, Shao R-G, Pommier Y. Assessment of DNA damage in apoptosis. Apoptosis. A practical approch. Oxford, Univ. press. 1999; 41-55.
[5]
Samuilov VD, Oleskin AV, Lagunova EM. Programmed cell death. Biochemistry (Mosc). 2000;65(8):873-87.
[6]
Peitsch MC, Muller C, Tschopp J. DNA fragmentation during apoptosis is caused by frequent single-strand cuts. Nucleic Acids Res. 1993; 21(18):4206-09.
[7]
Schwartz LM, Smith SW, Jones MEE, Osborne BA. Do all programmed cell deaths occur via apoptosis? Proc Nat Acad Sci USA. 1993; 90(3):980-4.
[8]
Fath A, Bethke PC, Jones RL. Barley aleurone cell death is not apoptotic: Characterization of nuclease activities and DNA degradation. Plant J. 1999; 20(3):305-15.
[9]
Mittler R, Lam E. Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death. Plant Cell. 1995; 7 (11):1951-62.
[10]
Mittler R, Simon L, Lam E. Pathogen-induced programmed cell death in tobacco. J Cell Sci. 1997; 110 (11):1333-44.
[11]
Kirnos MD, Aleksandrushkina HI, Shorning BYu, Kudryashova IB, Vanyushin BF. Internucleosomal fragmentation and DNA synthesis in wheat seedlings. Fiziologiia rasteniy. 1999; 46(1):48-57.
[12]
Xu Y, Hanson MR. Programmed cell death during pollination-induced petal senescence in petunia. Plant Physiol. 2000;122(4):1323-33.
[13]
Diagnosis of plant resistance to stress factors (Method Manual). Leningrad, WRI, 1988; 10-24.
[14]
Draper J, Scott R. Isolation of nucleic acids from plant cells. Genetic engineering of plants. Lab. Manual. Translated from Eng. New York, Wiley, 1991; 248-252.
[15]
Lobov VP, Tishchenko EN. Characteristics of sunflower genome by the reassociation kinetics method. Fiziologiia i biokhimiia kul'turnykh rasteniy. 1984; 16(3):256-61.
[16]
Britten RJ, Pavich M, Smith J. A new method for DNA purification. Carnegie Inst. Wash. Year Book. 1970; 68:400-402.
[17]
Osterman LA. Chromatography of proteins and nucleic acids. Moscow, Nauka, 1985; 221-248.
[18]
Quirino BF, Noh Y-S, Himelblau E, Amasino RM. Molecular aspects of leaf senescence. Trends Plant Sci. 2000; 5 (7):278-82.
[19]
Bjorklund S, Almouzni G, Davidson I, Nightingale KP, Weiss K. Global transcription regulators of eukaryotes. Cell. 1999; 96 (6):759-67.
[20]
Wolffe AP, Hayes JJ. Chromatin disruption and modification. Nucleic Acids Res. 1999; 27 (3):711-20.
[21]
Navarre DA, Wolpert TJ. Victorin induction of an apoptotic/senescence-like response in oats. Plant Cell. 1999; 11 (2):237-49.
[22]
Koukalova B, Kovarik A, Fajkus J, Siroky J. Chromatin fragmentation associated with apoptotic changes in tobacco cells exposed to cold stress. FEBS Lett. 1997; 414 (2):289-92.
[23]
Stein JC, Hansen G. Mannose induces an endonuclease responsible for DNA laddering in plant cells. Plant Physiol. 1999; 121 (1):71-9.
[24]
Danon A, Delorme V, Mailhac N, Gallois P. Plant programmed cell death: A common way to die. Plant Physiol Biochem. 2000; 38 (9):647-55.
[25]
Tishchenko EN, Daskalyuk TM, Mikhal'skaya SI. DNA degradation during senescence of soybean leaves. Dopovidi Nats Akad Nauk Ukrainy. 2003; (2):182-6.