Characterization of lipids A of Ralstonia solanacearum lipopolysaccharides

Varbanets, L. D.; Brovarskaya, O. S.; Vasiliev, V. N.; Vinarskaya, N. V.; Gogoman, I. V.

doi:10.7124/bc.0006C1

Biopolym. Cell. 2004; 20(5):398-401.

http://dx.doi.org/10.7124/bc.0006C1

Structure and Function of Biopolymers

Characterization of lipids A of Ralstonia solanacearum lipopolysaccharides

1Varbanets L. D., 1Brovarskaya O. S., 1Vasiliev V. N., 1Vinarskaya N. V., 1Gogoman I. V.

D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

The analysis of fatty acid profiles of lipopolysacharides has shown that R. solanacearum strains tested may be divided into two groups. The first group is represented by R. solanacearum strains (5712, 7945, 7955 and 8110) the lipids A of which contained hydroxylated fatty acids with long chains: 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic. The second group was represented by R. solanacearum strains the lipids A of which contained hydroxylated fatty acids with short chains: 3-hydroxydecanoic, 2-hydroxydodecanoic and 3-hydroxydodecanoic. 3-hydroxytetradecanoic acid was observed in a small amount. A comparative analysis of the fatty acid composition and biological activity gives a possibility to suppose that 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic acids may be responsible for the toxicity and pyrogenicity of the lipopolysaccharides tested.

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