Biopolym. Cell. 2004; 20(4):295-299.
Structure and Function of Biopolymers
Influence of Pseudomonas syringae pv. atrofaciens lipopolysaccharide on spontaneous and induced by bichromate potassium mutagenesis in Salmonella typhimurium
1Vashchenko L. M., 1Pasichnyk L. A., 1Gvozdyak R. I.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

It has been established that lipopolysaccharides (LPS) are not mutagens. LPS in concentration of 1000 g per plate reduced spontaneous mutations of S. typhimurium TA100 by 16 % and S. typhimurium TA98 by 31 %. P. syringae pv. atrofaciens 8281 LPS is the antimutagen against bichromate potassium too. LPS can reduce the frequency of induced by bichromate potassium mutations S. typhimurium TA98 by 57-78 %, S. typhimurium TA100 by 14- 49 %. P. syringae pv. atrofaciens 8281 LPS has neither stimulation nor toxic effect on S. typhimurium.

References

[1] Lukash L. L. Mutagenesis and antimutagenesis are contrary directed processes determining the level of genetic variability and stability. Biopolym. Cell. 1998; 14(6):500-511
[2] Musiyaka VK. Antimutagenic properties of natural origin substances. Fiziologiia i biokhimiia kul'turnykh rasteniy. 2001; 33(3):216-25.
[3] Vorob'yeva LI, Abilev SK. Antimutagenic properties of bacteria. Prikl Biokhim Mikrobiol. 2002;38(2):115-27.
[4] Vorob'eva LI, Cherdyntseva TA, Abilev SK. Antimutagenic action of bacterial culture liquid on mutagenesis induced by 2-nitrofluorene in Salmonella typhimurium strains. Genetika. 1995;31(7):901-7.
[5] Sreekumar O, Hosono A. The antimutagenic properties of a polysaccharide produced by Bifidobacterium longum and its cultured milk against some heterocyclic amines. Can J Microbiol. 1998;44(11):1029-36.
[6] Musiyaka V. K., Gladun A. A., Sarnackaya V. V., Gvozdyak R. I. Antimutagenic activity of Saccharomyces cerevisiae strains. Biopolym. Cell. 2000; 16(4):284-8
[7] Linder AV, Kabulova FD, Chernin LS, Ismailov ZF. Bioecological aspects of the interaction of soil microorganisms to eukaryotes. Dokl. Intern. Ecol. Congr. "New in ecology and life" (St. Petersburg, 14-16 June 2000). St. Petersburg, 2000; Vol. 2:395-7.
[8] Yakovleva LM. Role of bacterial glycopolymers in pathogenesis of plant bacterioses. Mikrobiol Zh. 1992; 54(3):87-102.
[9] Varbanets LD, Shmal'ko IuP, Pridatko OE, Zhukova EV, Moskalenko NV. The biological activity of Pseudomonas solanacearum polysaccharide. Mikrobiol Z. 1995;57(2):80-5.
[10] Vaara M. Lipopolysaccharide and the permeability of the bacterial outer membrane Endotoxin in helth and disease. Eds H. Brade et al. New York; Barsel: Marcel Dekker, Inc 1999;31-38.
[11] Zdorovenko GM, Yakovleva LM, Gvozdyak RI, Zakharova IYa, Koshechkina LN. Isolation, chemical composition and serological characterization of Pseudomonas wieringae polysaccharides. Mikrobiol Z. 1982; 44(4):65-70.
[12] Zakharova IYa, Kosenko L. Methods for studying microbial polysaccharides. Kiev, Naukova Dumka, 1982; 189 p.
[13] Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[14] Spirin AS. Spectrophotometric determination of total nucleic acids. Biokhimiia. 1958;23(5):656-62.
[15] Ames BN, Mccann J, Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res. 1975;31(6):347-64.
[16] McCann J, Spingarn NE, Kobori J, Ames BN. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci U S A. 1975;72(3):979-83.
[17] Pasichnik LA, Koroleva IB. Pseudomonas syringae pv. atrofaciens, an agent of bacterial spot of rye in the Ukraine. Mikrobiol Z. 1991; 53(2):49-55.
[18] Zakharova IYa, Tanatar NV. Lipopolysaccharides of Pseudomonasbacteria. . Mikrobiol Z. 1984; 46(6):78-92.
[19] Zhurkov VS, Dugan AM. Analysis of spontaneous background mutation in Salmonella typhimurium. Hygiene and sanitation. 1991; 1:70-1.
[20] Kalinina LM, Minseitova SR. Mutagenic and DNA-damaging effect of potassium bichromate in Escherichia coli cells. Genetika. 1983;19(12):1941-7.
[21] Hensel A, Meier K. Pectins and xyloglucans exhibit antimutagenic activities against nitroaromatic compounds. Planta Med. 1999;65(5):395-9.