Genetic determination of dissociation process of poliovirus type III

Kostenko, I. G.; Zhuravel, E. V.; Panasenko, G. V.; Shirobokov, V. P.

doi:10.7124/bc.0006AD

Biopolym. Cell. 2004; 20(3):255-258.

http://dx.doi.org/10.7124/bc.0006AD

Short Communications

Genetic determination of dissociation process of poliovirus type III

1Kostenko I. G., 1Zhuravel E. V., 1Panasenko G. V., 1Shirobokov V. P.

Bogomolets National Medical University
Blvd. Shevchenko, 13, Kyiv, Ukraine, 01601

Abstract

The variants of a vaccine virus strain of a poliomyelitis type III have been proved to occur in the course of reproduction in cell culture, and have differences in nucleotide sequences of a gene VP1. It has been demonstrated, that in variant A_bent+ the nucleotide adenine occupies position 2900, and in variant A_bent- - guanine. Accordingly, in the structure of protein VP1 an aspartic acid replaces asparagines in position 142, the former influencing the charge and conformation of virion surface proteins.

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References

[1] Harley A.R.Human enterovirus infections, Washington 1995; p. 95.

[2] Kornyushenko OM. Immunopathogenesis of experimental polio from the standpoint of population heterogeneity polioviruses: : Dis. Dr. ... biol. Sciences. Kiev, 1999; 333 p.

[3] Shirobokov VP. A comparative study of the biological properties of Coxsackie viruses and their variants were selected: Author. dis. ... Dr. med. Sciences. Kiev, 1977; 36 p.

[4] Mel'nik VV. Heterogeneity of Coxsackie B virus populations physical and chemical properties: Dis. ... Cand. med. Sciences. Kiev, 1996; 116 p.

[5] Shiprobokov VP, Zhurba TB, Zemlianskii VV. Properties of coxsackieviruses isolated from pancreatic tissue of patients with chronic pancreatitis. Mikrobiol Zh. 1988;50(4):78-81.

[6] Shirobokov VP. The physiological properties of Coxsackie virus with reference to their selective adsorption on bentonite. Mikrobiol Zh. 1972;34(1):122-3.

[7] Shyrobokov VP, Kostenko IH, Nikolaienko IV. Antigen differences of genetic variants Abent+ and Abent- poliovirus vaccine strain of III type. Mikrobiol Z. 2003;65(4):29-36.

[8] Shyrobokov VP, Kostenko IG. Differentiation bentonite options polioviruses using monoclonal antibodies. Journal of the Academy of Medical Sciences of Ukraine. 2003; 9 (4) 780-90.

[9] Kostenko IG. Rationale for new methodical approaches to obtain stable dysotsiantiv polioviruses. Molodizhnyy zh. 2002; 1:46-9 .

[10] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual New York: Cold Spring Harbor Lab. publ., 1982 545 p.

[11] Oblap RV. Differentiation gene pools of animals exposed to environmental stress factors: Dis. ... Cand. biol. Sciences. Kiev, 1998; 174 p.

[12] Lukashev AN Molecular epidemiological and pathogenetic study of 11 strains ECHOvirus 11 and ECHOvirus 19 - pathogens enterovirus outbreak of acute uveitis and sepsis-like disease in infants age: Dis. ... Cand. Med. Sciences. Moscow, 2002; 175 p.

[13] Morozov VM. Structure-activity analysis of amino acid sequences using the Internet. Mol Biol (Mosk). 1996;30(2):387-93.