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Structure and Function of Biopolymers
Isolation of biologically active poly(A)+ and poly (A)- mRNA from germinating wheat embryos
1Filimonov N. G., 1Agashkin A. O., 1Sablina O. D., 1Aitkhozhin M. A.
  1. Institute of Molecular Biology and Biochemistry, Academy of Sciences of the Kazakh SSR
    Alma-Ata, USSR


Biologically active poly (A) - mRNA were isolated from polyribosomes and free cytoplasmic informosomes of germinating wheat embryos by benzoylated cellulose chromato-graphy. Purified fractions of poly (A) - mRNA of polyribosomes and free isoformosomes do not essentially differ from cytoplasmic poly (A) + mRNA by molecular-weight characteristics and have translational activity in the cell-free system of protein synthesis. It is shown that poly (A) - mRNA of polyribosomes and free informosomes equally stimulate incorporation of [35S]methionine into acidinsoluble product, but the efficiency of their translation is much lower than that of poly (A)+ mRNA. The difference in the translation efficiency of cytoplasmic poly (A) + and poly (A) - mRNA of wheat embryos is obviously the metabolitic peculiarity of these mRNA and is not related to their isolation.


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