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Structure and Function of Biopolymers
Isolation of biologically active poly(A)+ and poly (A)- mRNA from germinating wheat embryos
- Institute of Molecular Biology and Biochemistry, Academy of Sciences of the Kazakh SSR
Biologically active poly (A) - mRNA were isolated from polyribosomes and free cytoplasmic informosomes of germinating wheat embryos by benzoylated cellulose chromato-graphy. Purified fractions of poly (A) - mRNA of polyribosomes and free isoformosomes do not essentially differ from cytoplasmic poly (A) + mRNA by molecular-weight characteristics and have translational activity in the cell-free system of protein synthesis. It is shown that poly (A) - mRNA of polyribosomes and free informosomes equally stimulate incorporation of [35S]methionine into acidinsoluble product, but the efficiency of their translation is much lower than that of poly (A)+ mRNA. The difference in the translation efficiency of cytoplasmic poly (A) + and poly (A) - mRNA of wheat embryos is obviously the metabolitic peculiarity of these mRNA and is not related to their isolation.
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