Biopolym. Cell. 1986; 2(5):275-278.
Insertion of a DNA fragment between two strong similarly oriented promoters of recombinant filamentous phage mp8t0/rpoB increases its stability and makes possible an opposite orientation of the cloned E. coli rpoB gene
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
A fragment of λ phage DNA containing terminator of transcription (t0) and terminal part of the oop-RNA gene was inserted into the polylinker area of M13 mp8 filamentous phage. The obtained phage was used to clone a BglII fragment of pJC703 cosmid, containing E. coli genes rpIJ, rpIL and rpoB together with promoters PJ and Pβ. Stability of the obtained mp8t /rpoB recombinant phages increased up to 93 % and an orientation of the cloned BglII-fragment opposite to the previously observed one became possible.
Full text: (PDF, in Russian)
 Paton EB, Vudmaska MI, Sverdlov ED. Unidirectional orientation of the rpo B gene of E. coli cloned into filamentous M13mp8 and M13WB2348 phages. Bioorg Khim. 1984;10(11):1544-7.
 Paton EB, Vudmaska MI, Sverdlov ED. Unidirectional orientation of the rpoB gene of E. coli, cloned in filamentous phage Ml3. Macromolecules in the functioning cell: Proc. of reports IV sympos. USSR - Italy. Kiev, 1984; 89.
 Paton EB, Woodmaska MI, Sverdlov ED. The stability of recombinant filamentous phages is reduced in the presence of the rpoB gene promoter. Biopolym Cell. 1985; 1(3):160-2.
 Gentz R, Langner A, Chang AC, Cohen SN, Bujard H. Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A. 1981;78(8):4936-40.
 Ovchinnikov YA, Guryev SO, Krayev AS, Monastyrskaya GS, Skryabin KG, Sverdlov ED, Zakharyev VM, Bayev AA. Primary structure of an EcoRI fragment of lambda imm434 DNA containing regions cI-cro of phage 434 and cII-o of phage lambda. Gene. 1979;6(3):235-49.
 Dretzen G, Bellard M, Sassone-Corsi P, Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981;112(2):295-8.
 Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning - a laboratory manual. New York : Cold Spring Harbor, 1982. 545 p.
 Miller JH. Experiments in molecular genetics. Cold Spring Harbor Laboratory, 1972, 466 p.
 Beck E, Zink B. Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd. Gene. 1981;16(1-3):35-58.
 Collins J. Deletions, insertions and rearrangements affecting rpoB gene expression. Mol Gen Genet. 1979;173(2):217-20.