Biopolym. Cell. 1986; 2(5):275-278.
Short Communications
Insertion of a DNA fragment between two strong similarly oriented promoters of recombinant filamentous phage mp8t0/rpoB increases its stability and makes possible an opposite orientation of the cloned E. coli rpoB gene
1Paton E. B., 1Zhivolup A. N.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


A fragment of λ phage DNA containing terminator of transcription (t0) and terminal part of the oop-RNA gene was inserted into the polylinker area of M13 mp8 filamentous phage. The obtained phage was used to clone a BglII fragment of pJC703 cosmid, containing E. coli genes rpIJ, rpIL and rpoB together with promoters PJ and Pβ. Stability of the obtained mp8t /rpoB recombinant phages increased up to 93 % and an orientation of the cloned BglII-fragment opposite to the previously observed one became possible.


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