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Insertion of a DNA fragment between two strong similarly oriented promoters of recombinant filamentous phage mp8t0/rpoB increases its stability and makes possible an opposite orientation of the cloned E. coli rpoB gene
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
A fragment of λ phage DNA containing terminator of transcription (t0) and terminal part of the oop-RNA gene was inserted into the polylinker area of M13 mp8 filamentous phage. The obtained phage was used to clone a BglII fragment of pJC703 cosmid, containing E. coli genes rpIJ, rpIL and rpoB together with promoters PJ and Pβ. Stability of the obtained mp8t /rpoB recombinant phages increased up to 93 % and an orientation of the cloned BglII-fragment opposite to the previously observed one became possible.
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