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Gene-Engineering Biotechnology
Transformation of Streptomyces griseus protoplasts by chromosomal and plasmid DNA incapsulated in lyposomes
1Stenko A. S., 1Matselyukh B. P., 1Dekhtyarenko T. D., 1Stephanishin E. E., 1Stefanov A. V., 1Bezkorovainaya N. K., 1Polishchuk L. V., 1Mashkovsky N. N.
  1. Institute of Microbiology and Virology, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


S. griseus protoplasts are transformed by chromosomal and plasmid DNA incapsulated in liposomes. The frequency of transformation by chromosomal markers of prototrophity and by plasmid markers of resistance to neomycin and the formation of growth inhibition zones by means of DNA incapsulated in liposomes was two-three orders higher in comparison with control DNA without liposomes. Plasmid DNA was isolated from transformants and remained stable for 6–7 generations, phenotype being unchanged for 12 generations.


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