Biopolym. Cell. 1986; 2(3):141-146.
Cell Biology
Stimulation of DNA synthesis in hepatocytes by serum fractions of hepatectomized rats
1Osipova L. A., 1Ivashchenko Yu. D., 1Nemly N. I., 1Bykorez A. I.
  1. R. E. Kavetsky Institute for Oncology Problems, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The acid-ethanol extract of the hepatectomized rat serum (obtained 3 or 24 hs after the partial hepatectomy) contains proteins stimulating DNA synthesis in the primary culture of the adult rat hepatocytes. The active fractions partially purified by gel-filtration on the bio-gel P-60 include the spectrum of proteins with a molecular weight of 50–70 kDalton characterized by a shift towards more acidic pH values in comparison with the control, as it was shown by electrofocusing electrophoresis. Stimulation of DNA synthesis in hepatocytcs in vitro is more pronounced under the effect of the active fractions of serum obtained in the earlier periods after the partial hepatectomy.


[1] Moolten FL, Bucher NL. Regeneration of rat liver: transfer of humoral agent by cross circulation. Science. 1967;158(3798):272-4.
[2] Fisher B, Szuch P, Levine M, Fisher ER. A portal blood factor as the humoral agent in liver regeneration. Science. 1971;171(3971):575-7.
[3] Morley CG, Kingdon HS. The regulation of cell growth. I. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats. Biochim Biophys Acta. 1973;308(2):260-75.
[4] Sakai A, Kountz SL. Stimulation of hepatocytes and lymphocytes in vitro by liver regeneration. Nature. 1975;257(5521):53-4.
[5] Nakamura T, Nawa K, Ichihara A. Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. Biochem Biophys Res Commun. 1984;122(3):1450-9.
[6] Michalopoulos G, Houck KA, Dolan ML, Leutteke NC. Control of hepatocyte replication by two serum factors. Cancer Res. 1984;44(10):4414-9.
[7] Labrecque DR, Wilson M, Fogerty S. Stimulation of HTC hepatoma cell growth in vitro by hepatic stimulator substance (HSS). Interactions with serum, insulin, glucagon, epidermal growth factor and platelet derived growth factor. Exp Cell Res. 1984;150(2):419-29.
[8] Higgins G, Anderson R. Experimental pathology of the liver: restoration of the liver of the white rats following partial surgical removal. Arch Pathol. 1931; 31:186-202.
[9] Roberts AB, Anzano MA, Lamb LC, Smith JM, Sporn MB. New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues. Proc Natl Acad Sci U S A. 1981;78(9):5339-43.
[10] Taylor JM, Mitchell WM, Cohen S. Characterization of the binding protein for epidermal growth factor. J Biol Chem. 1974;249(7):2188-94.
[11] Ivashchenko YuD, Osipova LA, Gut IT, Garmanchuk LV. Accelerated preparation of highly purified epidermal growth factor by liquid chromatography in reverse phase. Exp Oncol. 1985; 7(6):47-9.
[12] Grinchishin VP, Vinarchuk MP, Osipova LA. Obtaining isolated hepatocytes and their assessment. Tsitol Genet. 1981;15(2):21-6.
[13] Strom SC, Michalopoulos G. Collagen as a substrate for cell growth and differentiation. Methods Enzymol. 1982;82 Pt A:544-55.
[14] Shuklinov VA, Shumilina VV. Effect of various serum fractions on L-cell differentiation. Tsitologiia. 1982;24(12):1454-8.
[15] Russell WE, McGowan JA, Bucher NL. Partial characterization of a hepatocyte growth factor from rat platelets. J Cell Physiol. 1984;119(2):183-92.
[16] Iype PT, McMahon JB. Hepatic proliferation inhibitor. Mol Cell Biochem. 1984;59(1-2):57-80.