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Structure and Function of Biopolymers
The ionic strength dependence of the cooperativity factor in DNA melting
- Institute of Molecular Genetics, Academy of Sciences of the USSR
The melting temperatures for the d(AT)24·d(AT)24 stretch located inside the DNA helix or terminally have been determined in a wide range of the ionic strength values (0.01–1 M Na+). The values of cooperativity factor were calculated from the shifts in the melting temperature of the stretch due to its different boundary conditions. With a decrease of the sodium concentration from 1 to 0.01 M the cooperativity factor has dropped by three orders of magnitude, its change being less pronounced at high ionic strengths than at low ones.
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